TABLE OF CONTENTS
 

ABBREVIATIONS

LIST OF ORIGINAL PUBLICATIONS

INTRODUCTION

REVIEW OF THE LITERATURE

       Structure and regulation of sleep

       Association of GH secretion with sleep

       The effect of injected GH on sleep

       GH regulatory hypothalamic peptides

       Somatostatin cells in the CNS

       GHRH cells in the CNS

       Autoregulation of GH-axis in the brain

       The effect of injected GHRH on sleep

       The effect of injected somatostatin on sleep

       GH-secretagogues

       The role of galanin as a possible sleep regulating neuropeptide
 

AIMS OF THE STUDY

MATERIALS AND METHODS

       Experimental animals

       REM sleep deprivation

       Total sleep deprivation

       In situ hybridization protocol

       Cell count and computer densitometry

       Analysis of somatostatin cells

       Analysis of GHRH cells

       Analysis of galanin cells

       Plasma GH measurement during REM sleep deprivation

       Animal surgery for i.c.v. and local microinjections

       Injection procedure

       I.c.v. injections of somatostatin and somatostatin antagonist

       I.c.v. somatostatin antagonist after REM sleep deprivation

       I.c.v. injections of galanin

       Microinjections into locus coeruleus

       Sleep polygraphy

       Statistics
 

RESULTS

       The effect of REM and total sleep deprivation on somatostatin mRNA

       The effect of REM and total sleep deprivation on GHRH mRNA

       The effect of REM and total sleep deprivation on galanin mRNA

       The effect of REM sleep deprivation on plasma GH

   I.C.V. INJECTIONS OF SOMATOSTATIN, SOMATOSTATIN ANTAGONIST,  AND GALANIN

       Somatostatin and somatostatin antagonist

       Somatostatin antagonist after REM sleep deprivation

       Galanin

       Microinjections of somatostatin, somatostatin antagonist and galanin into the locus coeruleus
 

DISCUSSION

   GENERAL DISCUSSION

   THE POSSIBLE ROLE OF GHRH IN THE REGULATION OF SWS

       Paraventricular nucleus

       Arcuate nucleus

       Periventromedial hypothalamic area

   THE POSSIBLE ROLE OF SOMATOSTATIN IN THE REGULATION OF REM SLEEP

       Arcuate nucleus

       Periventricular nucleus

       Connections to locus coeruleus

       Injections of somatostatin and somatostatin antagonist

   CONTRIBUTION OF GALANIN IN SLEEP REGULATION

   CONTRIBUTION OF STRESS IN SLEEP DEPRIVATION EXPERIMENTS
 

CONCLUSIONS

SUMMARY

ACKNOWLEDGMENTS

REFERENCES
 
 

-> ABBREVIATIONS