LIST OF FIGURES

Figure 1. Communication between cells using signalling molecules

Figure 2. Classification of cytokines

Figure 3. RTKs that are receptors for growth factors

Figure 4. Example of indirect angiogenic activity: TGF-

Figure 5. Amino acid sequence alignment: hVEGF family members to hPDGF-A and B

Figure 6. VEGF family members and their receptors

Figure 7. Homologous exons and conserved cysteine residues in the VEGF family

Figure 8. Transgene detection by Southern blotting: EcoRI/EcoRV restriction fragments

Figure 9. Transposon-mediated insertion of VEGF-C cDNA into a baculovirus genome in E.coli

Figure 10. Allelic replacement in insect cells

Figure 11. Hematoxylin-eosin stained sections of K14-VEGF-B167 transgenic mouse and control littermate

Figure 12. K14-VEGF-C-FL transgenic mouse with littermate

Figure 13. Histological sections and electron microscopy of K14-VEGF-C-FL transgenic mouse skin

Figure 14. VEGF-C, FLT4 and FLK1 in situ hybridizations of K14-VEGF-C-FL transgenic mouse skin

Figure 15. Immunohistochemical analysis of K14-VEGF-C-FL transgenic mouse skin

Figure 16. Genomic organization of the human VEGF gene and mRNA splice variants

Figure 17. Genomic organization of the murine VEGF gene and mRNA splice variants

Figure 18. Genomic organization of the human VEGF-B gene and mRNA splice variants

Figure 19. Genomic organization of the murine VEGF-B gene and mRNA splice variants

Figure 20. Genomic organization of the human VEGF-C gene and mRNA splice variants

Figure 21. Genomic organization of the murine VEGF-C gene and mRNA splice variants

Figure 22. Exon/intron borders for human and murine VEGF

Figure 23. Exon/intron borders for human and murine VEGF-B

Figure 24. Exon/intron borders for human and murine VEGF-C

Figure 25. K14-VEGF-B167

Figure 26. K14-VEGF-B186

Figure 27. K14-VEGF-C-FL

Figure 28. K14-NC-VEGF-C-FL

Figure 29. -MHC-MHC-VEGF-B167

Figure 30. -MHC-VEGF-C-FL

Figure 31. RIP-VEGF-B167

Figure 32. RIP-VEGF-C-FL

Figure 33. pFB1-VEGF-B167

Figure 34. VEGF-B167-X3-pCRII

Figure 35. pVT-Bac-melSP-VEGF-B167

Figure 36. pFB1-melSP-VEGF-B167

Figure 37. pFB1-CHIS-VEGF-B186

Figure 38. pFB1-melSP-CHIS-VEGF-B186

Figure 39. pFB1-VEGF-C

Figure 40. VEGF-C-X3-pCI-neo

Figure 41. pVT-melSP-N-VEGF-C

Figure 42. pFB1-melSP-N-VEGF-C

Figure 43. pFB1-VEGF-C-SSV (short splice variant)

Figure 44. pFB1-melSP-N-VEGF-C-SSV (short splice variant)

Figure 45. pFB1-C-C1HIS-VEGF-C

Figure 46. pFB1-melSP-NC-C1HIS-VEGF-C

Figure 47. pFB1-C-C2HIS-VEGF-C

Figure 48. pFB1-melSP-NC-C2HIS-VEGF-C

Figure 49. pFB1-NC-C2HIS-VEGF-C

Figure 50. pFB1-C3HIS-VEGF-C

Figure 51. pFB1-melSP-C3HIS-VEGF-C

Figure 52. pFB1-NHIS-VEGF-C

Figure 53. Schematic representation of recombinant baculoviral VEGF-C proteins (selection)

Figure 54. VEGF165

Figure 55. VEGF-B167

Figure 56. VEGF-B167 (clone 23)

Figure 57. No release of VEGF-B167 upon heparin treatment

Figure 58. melSP-VEGF-B167

Figure 59. melSP-CHIS-VEGF-B186

Figure 60. Affinity purification of melSP-CHIS-VEGF-B186 from medium conditioned by HF cells

Figure 61. Metabolic labelling of VEGF-C

Figure 62. VEGF-C harvested 7 d p.i.

Figure 63. VEGF-C produced by Sf9 cells

Figure 64. VEGF-C produced by HF cells

Figure 65. melSP-N-VEGF-C

Figure 66. melSP-NC-C1HIS-VEGF-C and C-C1HIS-VEGF-C

Figure 67. Differently H6-tagged VEGF-C, reducing SDS-PAGE

Figure 68. Differently H6-tagged VEGF-C, non-reducing SDS-PAGE

Figure 69. Short splice variant of VEGF-C and melSP-N-VEGF-C

Figure 70. FLT4 stimulation with transfection supernatant

Figure 71. FLT4 and KDR stimulation with melSP-NC-C1HIS-VEGF-C

Figure 72. Stimulation of FLT4, comparison between Sf9 and HF cells

Figure 73. Titration of FLT4 stimulation with melSP-NC-VEGF-C

Figure 74. FLT4 stimulation with differently H6-tagged VEGF-C

Figure 75. Affinity purification of melSP-NC-C1HIS-VEGF-C

Figure 76. Comparison between Silver and Coomassie Brilliant Blue staining

Figure 77. -MHC-VEGF-B167 transgenic mice

Figure 78. K14-VEGF-C-FL transgenic mice