Browsing by Subject "activity"
Now showing items 1-3 of 3
-
(2021)During the brain development, GABAergic neurons, also referred as interneurons, migrate tangentially from the subpallium to the pallium. After intracortical dispersion, the interneurons start radial migration towards their final location in the cortex. Although the radial migration of interneurons is extensively studied, mechanisms guiding the migration remain relatively unknown. Here we studied how manipulation of cortical activity affects the radial migration and allocation of the cortical GABAergic neurons in the developing mouse brain. For this purpose, we utilized whisker trimming induced sensory deprivation in GAD67-GFP mice at postnatal days 2-5 (P2-P5) followed by cell counting in brain slices derived from P5 and P10-aged mice. In addition, we performed live-imaging of migrating neurons in organotypic cultures derived from P2 SST-TdTomato and 5HT3aR-GFP mice and cultured for 1 day in vitro. These two mouse lines roughly represent early- and late-born subpopulations of the GABAergic neurons. Live-imaging was accompanied by activity manipulations using different drugs and the Designer Receptors Exclusively Activated by Designer Drugs (DREADD) technology. Analysis of the interneurons’ allocation on the barrel cortex after the unilateral sensory deprivation revealed misallocation of GAD67+ neurons on deep cortical layers of the contralateral hemisphere of the ablation group at P5. Analysis of the tracks from the live-imaged migrating interneurons revealed altered saltatory movement behaviour of 5HT3aR+ interneurons when clozapine-N-oxide (CNO) was used to activate the electroporated GFP-GCaMP3-mCherry-hM3Dq neurons located on L2/3 of the cortex. Moreover, we observed reduced motility of migrating interneurons in the organotypic cultures treated with a KCC2 inhibitor that alters GABAA-receptor mediated transmission. Altogether, our results suggest that activity is important in promoting the radial migration of late-born interneurons during the first days of the postnatal development.
-
(2015)Objectives. Cleantech, as an emerging new industry, its development involves the influences from various aspects. As a small to medium-sized cleantech company, the activity in China can be very challenge to study holistically. This research is aimed to provide a holistic view of the challenges and possibilities of a small to medium-size cleantech company's activity in China from the expansive learning perspectives. The purpose of this research is threefold. Firstly, it analyzes the object and network to uncover the three historical phases of GreenStream's long trajectory leaning process. Secondly, it analyzes learning actions and contradictions to uncover the driving forces of development and the expansiveness of the activity. Thirdly, based on the historical analysis an analysis of expansive learning cycles is made, with the purpose to sketch the zone of proximal development across the learning cycles and address the future possibilities and challenges. Methods. The research case Company is GreenStream, a small to medium-sized Finnish cleantech company in China. 8 Interviewees were chose from the different parties involved in its China activity. 4 of the interviewees from the case company GreenStream, 1 interviewee from its Chinese partner, and 3 interviewees from the third parties. The data is firstly analyzed by the historical analysis, namely analysis of object of activity system and analysis of network, following the analysis of expansive learning cycles, namely analysis of learning steps and analysis of learning actions and analysis of developmental contradictions. The zone of proximal development is sketched based on the historical analysis and analysis of expansive learning cycles. Results and conclusions. Interesting findings emerge through the systematic analysis. Three historical analyses reveals the object and network transformed in 3 phases. The object has transformed in 3 historical phases with the unchanged main motivation, the evolving economic imperative, and other emerging forces from different element of the activity. The partnership activity emerged and consolidated with the network development. Object formation and network development thus form the criteria to divide the 3 historical phases from "Small Spark in China", to " Great Success in China" and then to "Standing on Two Feet in China". Analysis of expansive learning cycles reveals that the 3 historical phases contours 3 expansive learning cycles. The first learning cycle was disrupted, the second learning cycle completed and expanded, and the third learning cycle is under development. Each learning cycle has its own character, and within each learning cycle, different levels of contradictions emerged as the driving forces of the development of GreenStream. Through tracing different levels of contradictions through the 3 expansive learning cycles, the expansiveness of GreenStream's activity could be detected with regard to the expansion of the object and expansion over interruption. Object formation, partnership and policy over clean-tech industry become important three elements for the zone of proximal development of GreenStream's activity in China. Through the understanding of these dynamics and momentum of the China activity in the past and present by means of expansive learning aspects, the subject can better master and build the development of the activity system in the future.
-
(2010)One of the biggest challenges in Finland in the future will be the growing number of elderly people. It means that more and more people will be dependent on care and medication. According to research elderly people consider physical working order and an option for social interaction with other people to be the most important things for their well-being. As one ages the activity of living decreases. As a result other matters relating to the quality of life suffer as well. Older people are entitled to life worthy of a human being. To underpin this objective we have to maintain their independence and activity. This requires a certain level of health, well-being and working order which can all be influenced by other people. All activities are surrounded by some sort of an environment, so it is an important part of well-being. Green environment in particular is believed to have a positive effect to the well being and health. This is emphasized in hospitals and institutions. The first aim of this study was to map out the factors in green environment that motivate and encourage to use more time outdoors. The second aim was to study the impacts of the increased activity for the elderly people in the assisted living building Palta in Paimio, Finland. The research was carried out during summer 2009. Method was to observe the use of green environment and to interview nine habitants and their relatives and the staff of Paltanpuisto. The habitants were interviewed twice, spring and autumn. Other participants were interviewed once late summer. The object was to find out what were the reasons that got the habitants to go out and how did they feel it had affected their well-being The results were organized into three categories that were considered to activate habitants to go out: Safety, operationality and experientiality. The categories are interactive and in part overlapping. Experiences of well-being were considered as one category, which once again interacts with the three mentioned above. Main reasons to go out were the feeling of independence, other people and the affect going out has on general mood. In general this study confirms that green environment has a positive affect for the habitants of assisted living buildings. Most habitants saw the opportunity to go out as a vitally important factor behind good quality of life.
Now showing items 1-3 of 3