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Fluorescence Study of Nanoparticles Formed by Diblock Copolymer

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dc.date.accessioned 2019-06-24T07:59:07Z
dc.date.available 2019-06-24T07:59:07Z
dc.date.issued 2019-06-24
dc.identifier.uri http://hdl.handle.net/123456789/24724
dc.title Fluorescence Study of Nanoparticles Formed by Diblock Copolymer en
ethesis.discipline none und
ethesis.department none und
ethesis.faculty Matemaattis-luonnontieteellinen tiedekunta fi
ethesis.faculty Faculty of Science en
ethesis.faculty Matematisk-naturvetenskapliga fakulteten sv
ethesis.faculty.URI http://data.hulib.helsinki.fi/id/8d59209f-6614-4edd-9744-1ebdaf1d13ca
ethesis.university.URI http://data.hulib.helsinki.fi/id/50ae46d8-7ba9-4821-877c-c994c78b0d97
ethesis.university Helsingin yliopisto fi
ethesis.university University of Helsinki en
ethesis.university Helsingfors universitet sv
dct.creator Chou, Hao-Wei
dct.issued 2019
dct.language.ISO639-2 eng
dct.abstract Diblock copolymers, poly(lactide)-block-poly(2-isopropyl-2-oxazoline) (PiPOx-b-PLA) and n-octadecyl poly(2-isopropyl-2-oxazoline) (PiPOx-C18), were characterized using the steady-state fluorescence with two fluorescence probes, pyrene and (1,6-diphenyl-l,3,4-hexatriene) (DPH), to investigate the core structure of the particles formed by the polymers. The two enantiomers of PiPOx-b-PLA, which are PiPOx-PLLA and PiPOx-PDLLA, reflected very different features in terms of polarity of the micellar core and the partitioning equilibrium constants of pyrene (Kv). This suggests that the core of the particles formed by PiPOx-PDLLA has higher polarity due to high water content in the range of the polymer concentrations from 0.03 g/L to 3 g/L, and more pyrene molecules are bound to the particles. Furthermore, the core of the particles formed by PiPOx-PDLLA reveals rigidity lower than that of PiPOx-PLLA, which is supported by the low anisotropy value (r) of DPH. r did not change upon heating from 15 ⁰C to 65 ⁰C for the PiPOx-PLLA particles. A slight decrease of r occurred above 50 ⁰C for the PiPOx-PDLLA particle. The PiPOx-C18 micelles exhibit higher critical micellar concentration (CMC), smaller Kv, and softer core in comparison to the PiPOx-b-PLA micelles. These differences owe to the nature of the alkyl chain end. Loading and release of a hydrophobic drug, curcumin, to/from the PiPOx-PLLA and PiPOx-PDLLA micelles were investigated by means of the steady-state fluorescence. The release test was conducted using the dialysis bag method. The PiPOx-PDLLA particles encapsulated more curcumin with encapsulation efficiency (EE) value being 98% while the curcumin-to-polymer ratio was 1:20. Curcumin intake of the PiPOx-PLLA particles was lower under the same condition (EE = 56%). The PiPOx-PDLLA particles show excellent ability of trapping curcumin. PiPOx-PDLLA bear 70% of curcumin inside the dialysis bag after 50 h, whereas 40% of curcumin was discharged from dispersions of PiPOx-PLLA. Precipitation of curcumin was observed in presence of PiPOx-PLLA in 4 days. No curcumin precipitating from the PiPOx-PDLLA dispersion occurred and a decrease in the intensity of curcumin owed to the degradation of curcumin. Finally, all the phenomena observed are well illustrated by the hypothesis of the morphology of the particles formed by PiPOx-PLLA and PiPOx-PDLLA. The particle formed by PiPOx-PDLLA consists of a loose hydrophobic core with hydrated tails extending to the aqueous phase, whereas the core of PiPOx-PLLA particle is dense. en
dct.language en
ethesis.isPublicationLicenseAccepted false
ethesis.language.URI http://data.hulib.helsinki.fi/id/languages/eng
ethesis.language English en
ethesis.language englanti fi
ethesis.language engelska sv
ethesis.thesistype pro gradu -tutkielmat fi
ethesis.thesistype master's thesis en
ethesis.thesistype pro gradu-avhandlingar sv
ethesis.thesistype.URI http://data.hulib.helsinki.fi/id/thesistypes/mastersthesis
dct.identifier.ethesis E-thesisID:f907ff87-faf0-4a31-9003-e21a2f19bc2c
dct.identifier.urn URN:NBN:fi:hulib-201906243057
dc.type.dcmitype Text
ethesis.facultystudyline Polymeerimateriaalien kemia fi
ethesis.facultystudyline Polymer Materials Chemistry en
ethesis.facultystudyline Polymermaterialkemi sv
ethesis.facultystudyline.URI http://data.hulib.helsinki.fi/id/SH50_080
ethesis.mastersdegreeprogram Kemian ja molekyylitieteiden maisteriohjelma fi
ethesis.mastersdegreeprogram Master's Programme in Chemistry and Molecular Sciences en
ethesis.mastersdegreeprogram Magisterprogrammet i kemi och molekylära vetenskaper sv
ethesis.mastersdegreeprogram.URI http://data.hulib.helsinki.fi/id/MH50_007

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