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Engineering Cellular Communication Systems for Synthetic Biology

Show simple item record 2019-06-28T07:33:20Z 2019-06-28T07:33:20Z 2019-06-28
dc.title Engineering Cellular Communication Systems for Synthetic Biology en
ethesis.discipline biotekniikka fi
ethesis.discipline Biotechnology en
ethesis.discipline bioteknik sv
ethesis.department Bio- ja ympäristötieteellinen tiedekunta fi
ethesis.department Faculty of Biological and Environmental Sciences en
ethesis.department Bio- och miljövetenskapliga fakulteten sv
ethesis.faculty Bio- ja ympäristötieteellinen tiedekunta fi
ethesis.faculty Faculty of Biological and Environmental Sciences en
ethesis.faculty Bio- och miljövetenskapliga fakulteten sv
ethesis.faculty.URI Helsingin yliopisto fi University of Helsinki en Helsingfors universitet sv
dct.creator Kaya, Meryem Ecem
dct.issued 2019
dct.language.ISO639-2 eng
dct.abstract Synthetic biology is an emerging interdisciplinary field of biology that aims to system-atically design artificial biological systems. As synthetic biologists seek increasingly complex control over cellular processes to achieve robust and predictable systems. A new frontier in synthetic biology is engineering synthetic microbial consortia. This ap-proach employs the concept of division of labor, instead of introducing large genetic cir-cuitry to homogenous cell populations. In this approach, different cell types are assigned to execute a portion of the overall circuit. Each cell type communicates with their co-worker subpopulations to complete the circuit. The main advantage of this strategy is the reduced metabolic burden on each cell type. Thus, leading to more reliable and stable overall performance. In this work, to simplify cellular communication between the mem-bers of the consortium, we used the simple architecture of quorum sensing machinery. We constructed a toolbox that contains promoter, receptor and quorum sensing signal synthase genes along with fluorescent reporters. Using this toolbox, we constructed dif-ferent cell types that can be used in synthetic consortia forming various communication topologies. We characterized the constructed cell types individually and in co-cultures. en
dct.subject communication en
dct.subject consortia en
dct.subject quorum sensing en
dct.subject synthetic biology en
dct.language en
ethesis.language englanti fi
ethesis.language English en
ethesis.language engelska sv
ethesis.supervisor Linder, Markus
ethesis.supervisor Jonkergouw, Chris
ethesis.thesistype pro gradu -tutkielmat fi
ethesis.thesistype master's thesis en
ethesis.thesistype pro gradu-avhandlingar sv
dct.identifier.ethesis E-thesisID:61ed9dbf-f5f9-4fdb-8d43-c24eebc1b4e8
ethesis-internal.timestamp.reviewStep 2019-05-14 16:13:02:680
ethesis.principalprofessor Keinänen, Kari
dct.identifier.urn URN:NBN:fi:hulib-201906283094
dc.type.dcmitype Text
ethesis.facultystudyline.URI none
ethesis.mastersdegreeprogram.URI none

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