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The Effects Of Avian Influenza NS1 Protein On Interferon Promoter Activation

Show simple item record 2020-08-31T10:45:01Z 2020-08-31T10:45:01Z 2020-08-31
dc.title The Effects Of Avian Influenza NS1 Protein On Interferon Promoter Activation en
ethesis.discipline.URI none und
ethesis.faculty Bio- ja ympäristötieteellinen tiedekunta fi
ethesis.faculty Faculty of Biological and Environmental Sciences en
ethesis.faculty Bio- och miljövetenskapliga fakulteten sv
ethesis.faculty.URI Helsingin yliopisto fi University of Helsinki en Helsingfors universitet sv
dct.creator Pająk, Daria Anna
dct.issued 2020
dct.language.ISO639-2 eng
dct.abstract Influenza A viruses are pathogens infecting birds and selected mammals. They are responsible for around 500 000 human deaths each year and pose a substantial economic burden to the healthcare system. The most important pathway in influenza virus detection is a retinoic acid-inducible gene I pathway, which recognizes the 5’-triphosphate in viral RNA. Its activation leads to the production of interferons: a group of cytokines important in overcoming viral infection. In order to replicate successfully, viruses had to develop mechanisms to overcome host defences. They include, among others, regulation of interferons and interferon stimulated genes expression. During influenza A virus infection, this function is performed by viral non-structural protein 1 (NS1). The aim of this study was evaluating the effect of NS1 of five different avian influenza strains and one seasonal influenza strain on activation of type I and III interferon gene promoters. The NS1 of seasonal virus H3N2 shown the highest suppression of both interferon I and III promoters, while NS1 originating from avian H9N2 and H7N7 strains had limited effect on interferon promoter activation. NS1 of H5N1/04, H5N1/97 and H7N9 was very effective at suppressing interferon type I promoter, which correlates with the severity of the infection in humans. When it comes to interferon type III promoter, H7N9 was very efficient at the suppression, while NS1 of H5N1/04 had little impact on promoter activation. The study has provided more information on the efficiency of potentially pandemic avian influenza strains at inhibition of interferon response and may be a base for further research. The project was conducted at the Finnish Institute of Health and Welfare. en
dct.subject avian influenza en
dct.subject H5N1 en
dct.subject H7N9 en
dct.subject H9N2 en
dct.subject H7N7 en
dct.subject H3N2 en
dct.subject interferons en
dct.subject RIG-I en
dct.subject NS1 en
dct.language en
ethesis.language English en
ethesis.language englanti fi
ethesis.language engelska sv
ethesis.supervisor Österlund, Pamela
ethesis.thesistype pro gradu -tutkielmat fi
ethesis.thesistype master's thesis en
ethesis.thesistype pro gradu-avhandlingar sv
dct.identifier.ethesis E-thesisID:3c8ebe15-8222-44e9-b00f-ef6a5396cb52
ethesis-internal.timestamp.reviewStep 2020-06-12 14:01:29:322
ethesis.principalprofessor Siljander, Pia
dct.identifier.urn URN:NBN:fi:hulib-202008313946
dc.type.dcmitype Text
ethesis.facultystudyline Molekulaaristen ja analyyttisten terveyden biotieteiden opintosuunta fi
ethesis.facultystudyline Molecular and analytical health biosciences study track en
ethesis.facultystudyline Molekylära och analytiska hälsans biovetenskap studieinriktning sv
ethesis.facultystudyline.URI und
ethesis.mastersdegreeprogram Genetiikan ja molekulaaristen biotieteiden maisteriohjelma fi
ethesis.mastersdegreeprogram Master's Programme in Genetics and Molecular Biosciences en
ethesis.mastersdegreeprogram Magisterprogrammet i genetik och molekylära biovetenskaper sv
ethesis.mastersdegreeprogram.URI und

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