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The role of LITTLE ZIPPER (ZPR) genes in the regulation of root vascular development

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dc.date.accessioned 2022-06-17T05:22:16Z
dc.date.available 2022-06-17T05:22:16Z
dc.date.issued 2022-06-17
dc.identifier.uri http://hdl.handle.net/123456789/41759
dc.title The role of LITTLE ZIPPER (ZPR) genes in the regulation of root vascular development en
ethesis.faculty Maatalous-metsätieteellinen tiedekunta fi
ethesis.faculty Faculty of Agriculture and Forestry en
ethesis.faculty Agrikultur- och forstvetenskapliga fakulteten sv
ethesis.faculty.URI http://data.hulib.helsinki.fi/id/b197f237-5f76-4651-a885-b5b849512034
ethesis.university.URI http://data.hulib.helsinki.fi/id/50ae46d8-7ba9-4821-877c-c994c78b0d97
ethesis.university Helsingin yliopisto fi
ethesis.university University of Helsinki en
ethesis.university Helsingfors universitet sv
dct.creator Pandey, Kailash
dct.issued 2022 xx
dct.abstract A well-adjusted regulation of vascular development is crucial for plant growth and development. CLASS III HOMEODOMAIN LEUCINE ZIPPER (HD-ZIP III) genes play a decisive role in cambial stem-cell maintenance, xylem cell fate determination, and ensuring proper patterning of vasculature. LITTLE ZIPPER (ZPR) microproteins are involved in the post-translational regulation of HD-ZIP III via a negative feedback mechanism. The protein-protein docking has predicted that HD-ZIP III and ZPR interact at the b-ZIP domain, which is an evolutionarily conserved region. The expression and mutant phenotype of ZPR in root vasculature haven’t been previously described. In this study, we developed the reporter lines and checked the expression in the Arabidopsis root. ZPR1 and ZPR3 showed a distinct and continuous expression from root tip to elongation and differentiation zone. In the cross-section, the expression was detected in cambium and xylem cells. Unlike ZPR1, ZPR3 showed a stronger expression towards the phloem side. Although ZPR2 didn’t show any endogenous expression, expression was detected in the primary root tip and lateral root initiation site upon xylemin treatment. Exogenous auxin application increased the expression of ZPR3, but it required a longer response time. The expression results suggest that ZPR follows the HD-ZIP III expression pattern, however, each ZPR shows some variation from the others. In the mutant analysis, zpr1 showed increased cellular proliferation in the vasculature, but zpr2 had no difference compared to the wild type. The results suggest that ZPR and HD-ZIP III have their function in a similar region in the root vasculature. However, further empirical evidence is necessary to elucidate the interaction of HD-ZIP III and ZPR, and its role in the regulation of root vascular development. en
dct.subject ZPR
dct.subject HD-ZIP III
dct.subject root
dct.subject vasculature
dct.subject cambium
ethesis.language.URI http://data.hulib.helsinki.fi/id/languages/eng
ethesis.language englanti fi
ethesis.language English en
ethesis.language engelska sv
ethesis.supervisor Prof. Dr. Ari Pekka Mähönen, Dr. Brecht Wybouw und
ethesis.thesistype pro gradu -tutkielmat fi
ethesis.thesistype master's thesis en
ethesis.thesistype pro gradu-avhandlingar sv
ethesis.thesistype.URI http://data.hulib.helsinki.fi/id/thesistypes/mastersthesis
dct.identifier.ethesis E-thesisID:8b805c37-b87c-4e3f-b34e-99077080345e
ethesis-internal.timestamp.reviewStep 2022-05-06 08:13:43:939
ethesis.principalprofessor Prof. Dr. Teemu Teeri und
dct.identifier.urn URN:NBN:fi:hulib-202206172815
ethesis.facultystudyline Kasvintuotantotieteet fi
ethesis.facultystudyline Plant production sciences en
ethesis.facultystudyline Växtproduktionsvetenskap sv
ethesis.facultystudyline.URI http://data.hulib.helsinki.fi/id/SH80_106
ethesis.mastersdegreeprogram Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) fi
ethesis.mastersdegreeprogram Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) en
ethesis.mastersdegreeprogram Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) sv
ethesis.mastersdegreeprogram.URI http://data.hulib.helsinki.fi/id/MH80_008

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