| dc.date.accessioned |
2022-06-17T05:23:00Z |
|
| dc.date.available |
2022-06-17T05:23:00Z |
|
| dc.date.issued |
2022-06-17 |
|
| dc.identifier.uri |
http://hdl.handle.net/123456789/41761 |
|
| dc.title |
Fine mapping of genes determining vicine-convicine concentration in faba bean |
en |
| ethesis.faculty |
Maatalous-metsätieteellinen tiedekunta |
fi |
| ethesis.faculty |
Faculty of Agriculture and Forestry |
en |
| ethesis.faculty |
Agrikultur- och forstvetenskapliga fakulteten |
sv |
| ethesis.faculty.URI |
http://data.hulib.helsinki.fi/id/b197f237-5f76-4651-a885-b5b849512034 |
|
| ethesis.university.URI |
http://data.hulib.helsinki.fi/id/50ae46d8-7ba9-4821-877c-c994c78b0d97 |
|
| ethesis.university |
Helsingin yliopisto |
fi |
| ethesis.university |
University of Helsinki |
en |
| ethesis.university |
Helsingfors universitet |
sv |
| dct.creator |
Laugel, Henri |
|
| dct.issued |
2022 |
|
| dct.abstract |
Faba bean (Vicia faba L.) is an annual herbaceous cool-season food legume widely cultivated worldwide, especially for its high seed protein content. However, its major limitation in being used as food and feed, is the presence of antinutritional factors in its seeds, especially vicine and convicine (VC), two related compounds, which may be harmful to livestock and G6PD-deficient humans. To remove VC, the most sustainable method is breeding for low-VC faba bean cultivars. To improve the efficiency and speed of breeding programs, breeders use marker-assisted selection (MAS). The identification of genes responsible for VC content allows the development of reliable DNA markers and a better understanding of the molecular basis of this trait. The major-effect QTL controlling VC content named “VC1”, was identified in faba bean chromosome 1, and a few minor-effect QTLs were detected in previous studies. Hence, a total of 165 RILs from the cross Mélodie/2 (low-VC) x ILB 938/2 (high-VC) were genotyped and evaluated for VC content. Composite interval mapping was run on R/qtl software with accurate phenotypic data associated with a high-density SNP-based genetic map. Results revealed two minor-effect QTLs in addition to VC1. One was on chromosome 4 and had about 15% effect on convicine content. The other was on chromosome 5 and had 15% effect on vicine and total VC content. This research also reports candidate genes for the newly detected minor-effect QTLs through comparative genomics with the Medicago truncatula genome. Hypotheses were proposed on the role of these candidate genes on the VC biosynthetic pathway or transportation into the embryo beans for further testing. |
en |
| dct.subject |
Faba bean |
|
| dct.subject |
vicine |
|
| dct.subject |
convicine |
|
| dct.subject |
QTL |
|
| dct.subject |
linkage mapping |
|
| dct.subject |
candidate gene identification |
|
| ethesis.language.URI |
http://data.hulib.helsinki.fi/id/languages/eng |
|
| ethesis.language |
englanti |
fi |
| ethesis.language |
English |
en |
| ethesis.language |
engelska |
sv |
| ethesis.supervisor |
Dr. Frederick Stoddard and Dr. Hamid Khazaei |
und |
| ethesis.thesistype |
pro gradu -tutkielmat |
fi |
| ethesis.thesistype |
master's thesis |
en |
| ethesis.thesistype |
pro gradu-avhandlingar |
sv |
| ethesis.thesistype.URI |
http://data.hulib.helsinki.fi/id/thesistypes/mastersthesis |
|
| dct.identifier.ethesis |
E-thesisID:fd11032f-f28a-4a6e-bb38-dcd7f425ab09 |
|
| ethesis-internal.timestamp.reviewStep |
2022-05-12 15:22:37:470 |
|
| ethesis.principalprofessor |
Teemu Teeri |
und |
| dct.identifier.urn |
URN:NBN:fi:hulib-202206172817 |
|
| ethesis.facultystudyline |
Kasvintuotantotieteet |
fi |
| ethesis.facultystudyline |
Plant production sciences |
en |
| ethesis.facultystudyline |
Växtproduktionsvetenskap |
sv |
| ethesis.facultystudyline.URI |
http://data.hulib.helsinki.fi/id/SH80_106 |
|
| ethesis.mastersdegreeprogram |
Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) |
fi |
| ethesis.mastersdegreeprogram |
Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) |
en |
| ethesis.mastersdegreeprogram |
Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) |
sv |
| ethesis.mastersdegreeprogram.URI |
http://data.hulib.helsinki.fi/id/MH80_008 |
|
