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Fine mapping of genes determining vicine-convicine concentration in faba bean

Show simple item record 2022-06-17T05:23:00Z 2022-06-17T05:23:00Z 2022-06-17
dc.title Fine mapping of genes determining vicine-convicine concentration in faba bean en
ethesis.faculty Maatalous-metsätieteellinen tiedekunta fi
ethesis.faculty Faculty of Agriculture and Forestry en
ethesis.faculty Agrikultur- och forstvetenskapliga fakulteten sv
ethesis.faculty.URI Helsingin yliopisto fi University of Helsinki en Helsingfors universitet sv
dct.creator Laugel, Henri
dct.issued 2022
dct.abstract Faba bean (Vicia faba L.) is an annual herbaceous cool-season food legume widely cultivated worldwide, especially for its high seed protein content. However, its major limitation in being used as food and feed, is the presence of antinutritional factors in its seeds, especially vicine and convicine (VC), two related compounds, which may be harmful to livestock and G6PD-deficient humans. To remove VC, the most sustainable method is breeding for low-VC faba bean cultivars. To improve the efficiency and speed of breeding programs, breeders use marker-assisted selection (MAS). The identification of genes responsible for VC content allows the development of reliable DNA markers and a better understanding of the molecular basis of this trait. The major-effect QTL controlling VC content named “VC1”, was identified in faba bean chromosome 1, and a few minor-effect QTLs were detected in previous studies. Hence, a total of 165 RILs from the cross Mélodie/2 (low-VC) x ILB 938/2 (high-VC) were genotyped and evaluated for VC content. Composite interval mapping was run on R/qtl software with accurate phenotypic data associated with a high-density SNP-based genetic map. Results revealed two minor-effect QTLs in addition to VC1. One was on chromosome 4 and had about 15% effect on convicine content. The other was on chromosome 5 and had 15% effect on vicine and total VC content. This research also reports candidate genes for the newly detected minor-effect QTLs through comparative genomics with the Medicago truncatula genome. Hypotheses were proposed on the role of these candidate genes on the VC biosynthetic pathway or transportation into the embryo beans for further testing. en
dct.subject Faba bean
dct.subject vicine
dct.subject convicine
dct.subject QTL
dct.subject linkage mapping
dct.subject candidate gene identification
ethesis.language englanti fi
ethesis.language English en
ethesis.language engelska sv
ethesis.supervisor Dr. Frederick Stoddard and Dr. Hamid Khazaei und
ethesis.thesistype pro gradu -tutkielmat fi
ethesis.thesistype master's thesis en
ethesis.thesistype pro gradu-avhandlingar sv
dct.identifier.ethesis E-thesisID:fd11032f-f28a-4a6e-bb38-dcd7f425ab09
ethesis-internal.timestamp.reviewStep 2022-05-12 15:22:37:470
ethesis.principalprofessor Teemu Teeri und
dct.identifier.urn URN:NBN:fi:hulib-202206172817
ethesis.facultystudyline Kasvintuotantotieteet fi
ethesis.facultystudyline Plant production sciences en
ethesis.facultystudyline Växtproduktionsvetenskap sv
ethesis.mastersdegreeprogram Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) fi
ethesis.mastersdegreeprogram Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) en
ethesis.mastersdegreeprogram Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) sv

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