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Insights on mechanical transmission of turnip mosaic virus (TuMV) mutants in Nicotiana benthamiana

Show simple item record 2022-06-17T05:26:23Z 2022-06-17T05:26:23Z 2022-06-17
dc.title Insights on mechanical transmission of turnip mosaic virus (TuMV) mutants in Nicotiana benthamiana en
ethesis.faculty Maatalous-metsätieteellinen tiedekunta fi
ethesis.faculty Faculty of Agriculture and Forestry en
ethesis.faculty Agrikultur- och forstvetenskapliga fakulteten sv
ethesis.faculty.URI Helsingin yliopisto fi University of Helsinki en Helsingfors universitet sv
dct.creator Jyakhwa, Sarkal
dct.issued 2022
dct.abstract Turnip mosaic virus (TuMV) is an economically important plant RNA virus causing huge damage to wide range of arable and vegetable crops. A study was conducted in Nicotiana benthamiana to know if a TuMV mutant carrying a mutation in a thoroughly conserved WD-domain interacting motif and WG motif in HCPro protein can be mechanically transmitted to a healthy plant or not. HCPRoWD is a mutation in “AELPR” motif where glutamic acid and arginine are replaced by alanine. This mutated virus is here referred as TuMVWD. Similarly, in TuMVAG the tryptophan residue in the WG pair is changed to alanine and this mutated HCPro is called as TuMVAG. Four treatments, TuMVWT (positive control), Mock (negative control), TuMVWD and TuMVAG were made. Three plants per treatment were agroinfiltrated and five plants per treatment were used for mechanical inoculation experiment. Green fluorescent protein (GFP), a quantitative reporter of gene expression, was measured followed by qPCR for quantification of vRNA (viral RNA) accumulation. In agroinfiltrated plants, newly emerged leaves showed strong fluorescence in TuMVWT and TuMVAG by 14 dpi (days post inoculation), but TuMVWD showed poor GFP as compared to TuMVWT. During mechanical inoculation experiment, none of the treatments developed GFP in systemic leaves by six dpi but by 14 dpi GFP accumulation in the upper leaves of TuMVWT and TuMVAG was increased. TuMVWD was not used for 2nd mechanical experiment as it did not cause systemic infection during 1st mechanical inoculation experiment even by 14 dpi. Results from vRNA accumulation showed that mechanical transmission of virus was reduced with TuMVAG and not possible with TuMVWD. However, mutations had negative effect on vRNA accumulation. en
dct.subject Keywords Potyvirus
dct.subject agroinfiltration
dct.subject mechanical inoculation
dct.subject vRNA
dct.subject qPCR
dct.subject systemic infection.
ethesis.language englanti fi
ethesis.language English en
ethesis.language engelska sv
ethesis.supervisor Dr. Kristiina Maria Makinen and Dr. Maija Pollari und
ethesis.thesistype pro gradu -tutkielmat fi
ethesis.thesistype master's thesis en
ethesis.thesistype pro gradu-avhandlingar sv
dct.identifier.ethesis E-thesisID:f3b0d0a3-1fd3-4251-887d-7332ac6f8b84
ethesis-internal.timestamp.reviewStep 2022-05-12 15:55:38:894
ethesis.principalprofessor Dr. Kristiina Maria Makinen und
dct.identifier.urn URN:NBN:fi:hulib-202206172823
ethesis.facultystudyline Kasvintuotantotieteet fi
ethesis.facultystudyline Plant production sciences en
ethesis.facultystudyline Växtproduktionsvetenskap sv
ethesis.mastersdegreeprogram Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) fi
ethesis.mastersdegreeprogram Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) en
ethesis.mastersdegreeprogram Erasmus-Mundus Master's Programme in Plant Breeding (EMPLANT) sv

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