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RNAscope RNA in situ hybridisation analysis of potential stemness-related biomarkers in high-grade serous ovarian carcinoma

Show simple item record 2022-08-31T06:35:03Z 2022-08-31T06:35:03Z 2022-08-31
dc.title RNAscope RNA in situ hybridisation analysis of potential stemness-related biomarkers in high-grade serous ovarian carcinoma en
ethesis.faculty Bio- ja ympäristötieteellinen tiedekunta fi
ethesis.faculty Faculty of Biological and Environmental Sciences en
ethesis.faculty Bio- och miljövetenskapliga fakulteten sv
ethesis.faculty.URI Helsingin yliopisto fi University of Helsinki en Helsingfors universitet sv
dct.creator Raineva, Iona
dct.issued 2022
dct.abstract High-grade serous carcinoma (HGSC) is the most common and lethal subtype of ovarian cancer. To date, HGSC has typically been diagnosed late, and the survival rate is poor. Relapses are common despite standardised treatment options, and platinum-based chemotherapy resistance remains frequent. The tumours are generally heterogeneous, which makes HGSC complex. Molecular mechanisms of tumour initiation, progression and chemoresistance are insufficiently understood. Thus, efficacious treatment is challenging, and current options do not help some patients. In addition, a population of cancer cells having stem cell-like properties are suggested to play a role in tumour initiation, progression and chemoresistance. There is an urgent need to better understand prognostic biomarkers and treatment responses in HGSC. With diverse analytical methods, the treatment-unresponsive patients and their outcomes could be identified by predictive biomarkers. This thesis aimed to validate potential tissue biomarkers associated with cancer stem cells and poor prognosis in HGSC, evaluate the usability of the RNAscope technique and briefly review the hypotheses on cancer stem cells. Four putative biomarkers were studied: ALDH1A1, BMI1, MYC and SOX2. The RNAscope technique was used to detect and quantify the biomarker expression. Using diagnostic tumour tissue specimens from 95 patients allowed capturing the expression in situ. With comprehensive clinical information, we could test whether the biomarkers distinguished patients with similar background information but different outcomes. This thesis shows that BMI1 could be a potential prognostic biomarker in high-grade serous carcinoma. The results provide information about the expression patterns of previously identified potential HGSC stemness markers. Proteomics studies such as immunohistochemistry could provide complementary information. When the molecular mechanisms and prognostic markers are better understood, they will provide a promising opportunity to develop novel diagnostic methods for predicting the outcomes and deciding treatments for this complex disease. en
dct.subject ovarian cancer
dct.subject high-grade serous ovarian carcinoma
dct.subject RNA in situ hybridisation
dct.subject RNAscope
dct.subject cancer stem cells
dct.subject BMI1
ethesis.language englanti fi
ethesis.language English en
ethesis.language engelska sv
ethesis.supervisor Olli Carpén, Satu Hänninen und
ethesis.thesistype pro gradu -tutkielmat fi
ethesis.thesistype master's thesis en
ethesis.thesistype pro gradu-avhandlingar sv
dct.identifier.ethesis E-thesisID:00fc5d93-b1ea-484a-9da7-f941f9bbadf8
ethesis-internal.timestamp.reviewStep 2022-06-20 13:21:39:656
ethesis.principalprofessor Pia Siljander und
dct.identifier.urn URN:NBN:fi:hulib-202208313403
ethesis.facultystudyline Molecular and Analytical Health Biosciences fi
ethesis.facultystudyline Molecular and Analytical Health Biosciences en
ethesis.facultystudyline Molecular and Analytical Health Biosciences sv
ethesis.mastersdegreeprogram Genetiikan ja molekulaaristen biotieteiden maisteriohjelma fi
ethesis.mastersdegreeprogram Master's Programme in Genetics and Molecular Biosciences en
ethesis.mastersdegreeprogram Magisterprogrammet i genetik och molekylära biovetenskaper sv

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