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Dynamics of fetal-like reparative state in the intestinal epithelium ex vivo

Show simple item record 2023-01-25T07:37:02Z 2023-01-25
dc.title Dynamics of fetal-like reparative state in the intestinal epithelium ex vivo en
ethesis.faculty Lääketieteellinen tiedekunta fi
ethesis.faculty Faculty of Medicine en
ethesis.faculty Medicinska fakulteten sv
ethesis.faculty.URI Helsingin yliopisto fi University of Helsinki en Helsingfors universitet sv
dct.creator Nestaite, Ernesta
dct.issued 2023 und
dct.abstract Intestinal epithelium is capable of rapid regeneration, which is associated with transient changes in cellular identity. Some of these changes involve an enrichment of fetal-like gene expression and simultaneous suppression of adult stem cell signature. Interestingly, the upregulation of fetal-like marker Stem cell antigen 1 (Sca1) is modulated by extracellular matrix (ECM) which is known to guide epithelial cells during regeneration. Our recently published decellularized small intestinal ECM (iECM) system retains the composition and topology of natural ECM. This makes it an attractive system for ex vivo studies addressing regeneration. This thesis aimed to gain insight into the fetal-like identity and its dynamics using an ex vivo iECM system. Intriguingly, Sca1 expressing cells on iECM displayed migratory features, such as a leading edge and changes in nuclear morphology. Curiously, these features are typical for epithelial cells during development. Furthermore, based on marker gene expression during iECM re-epithelization, fetal-like state was upregulated while adult stem cell state was downregulated, revealing a gradually emerging inverse correlation. Additionally, data suggests that circadian rhythms may have a role in modulating the fetal-like state. iECM from an active-state mice indicated a reduced capability to induce fetal-like identity and overall re-epithelization compared to the rest-state iECM. The results of this thesis suggest further potential of iECM system in studying emergence of fetal-like state during re-epithelization and circadian rhythm impact on it. en
dct.subject fetal-like
dct.subject intestine
dct.subject regeneration
ethesis.language englanti fi
ethesis.language English en
ethesis.language engelska sv
ethesis.supervisor Pekka Katajisto und
ethesis.thesistype pro gradu -tutkielmat fi
ethesis.thesistype master's thesis en
ethesis.thesistype pro gradu-avhandlingar sv
dct.identifier.ethesis E-thesisID:f594cd75-4207-48fe-b68f-2d458169fe33 2024-01-24
ethesis-internal.timestamp.reviewStep 2022-12-02 10:18:44:465
dct.identifier.urn URN:NBN:fi:hulib-202301251101 Intestinal regeneration und
ethesis.facultystudyline Regenerative medicine fi
ethesis.facultystudyline Regenerative medicine en
ethesis.facultystudyline Regenerative medicine sv
ethesis.mastersdegreeprogram Translationaalisen lääketieteen maisteriohjelma (Translational Medicine) fi
ethesis.mastersdegreeprogram Master's Programme in Translational Medicine en
ethesis.mastersdegreeprogram Magisterprogrammet i translationell medicin sv

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