Browsing by Subject "rypsi"

Increasing crop yield is one of the objectives of plant breeding to turnip rape (Brassica rapa subsp. oleifera), which could be achieved with hybrid breeding method. Most promising method for turnip rape is the Ogu-INRA cms / Rf hybrid seed production method, which transferred from Japanese radish (Raphanus sativus) to rapeseed (Brassica napus subsp. oleifera). The hybrid breeding method for turnip rape still lacks a fertility restorer paternal line and solution has been sought through interspecific crosses between turnip rape and rapeseed. Turnip rape, rapeseed and radish contain similar regions in their genetic material, which allows the transfer of radish’s fertil-ity restoring PPR-gene region from rapeseed to turnip rape. The aim of this Master’s thesis was to develop an SNP-tool for the identification of R9 chromo-some regions and using SNP-tool to identify turnip rape F1 hybrid offsprings, which have fertility restoring PPR-B gene region, integrated from radish extra chromosome into the turnip rape ge-nome. Earlier in the project PPR-gene was located to the R9 chromosome with BAC64-clone, therefore work focused solely on identifying R9 chromosome regions. For the development of the SNP-tool, objective was to search for a database and select species-specific SNP-markers with in silico-method. B.rapa databases were selected for the work and SNP-markers species specificity tested in the laboratory. The developed SNP-tool consisted of species-specific 28 radish SNP-markers and 48 turnip rape SNP-markers. The results showed amplification of four radish SNP-markers in fertile indi-viduals of turnip rape offspring, which located closest to the PPR-B gene region that restores fertility on the R9 chromosome. However, the SNP-tool could not determine whether integration occurred in fertile individuals of F1 hybrid offsprings, or whether the fertility restoring PPR-B gene region was unintegrated radish chromosome, as all SNP-tools turnip rape SNP-markers am-plified in the turnip rape offsprings. The SNP tool cannot be utilized to develop turnip rape hybrid breeding method, because screening of integration would done to turnip rape F1 hybrids. In fertile individuals amplificated radish SNP-markers could be used as DNA selection markers to identify the individuals with the PPR-B gene region, that restores fertility in F1 hybrids. In addition, the SNP-tool revealed, that the excess of extra radish chromosome is not complete in the genome. This research is part of a research project of the development of a hybrid breeding method for turnip rape, which is studying the utilization of cross breeding between closely related plant spe-cies in the breeding to turnip rape in cooperation with Boreal Plant Breeding Ltd.