Browsing by Subject "tyvi- ja märkämätä"

Bacterial soft rot and blackleg are amongst the major diseases of potato (Solanum tu-berosum L.). In Finland Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. carotovorum, Pectobacterium wasabiae and Dickeya-species cause rotting of the tuber and softening of stems on potato. These diseases cause major postharvest losses and weaken the quality of the crop, which is a problem in seed potato production. The aim of this study was to determine whether the antagonistic bacteria are toxic to in vitro plants, as well as to study the ability of the antagonistic bacteria to spread inside or on the in vitro plants. It was also investigated if the antagonistic bacteria can prevent soft rot of potato in in vitro plants, as well as the ability of the antagonistic bacteria to prevent the spreading of soft rot bacteria from soil to the plants. This study was carried out in laboratory and in greenhouse conditions in the University of Helsinki at the Department of Agricultural Sciences. As potato material cultivar Melody was used and as antagonistic bacteria 10 different bacteria were chosen: Serratia plymuthica A30 (NR), Flavobacterium sp. strain B17 (A3), Agrobacterium sp. (A6), unsequensed bacteria isolated from potato root (A4), Serratia j5h9 (S1), Alcaligenes faecalis (R8), Bacillus M1ju27 (BC1), Bacillus S3H53 (BC2), Burkholderia M1423 (BK3) and Pseudomonas S2h50 (PS3). Dickeya solani and Pectobacterium carotovorum were used as pathogens. Although BC1, BC2, S1 and BK3 antagonists did not influence negatively the potato in vitro seedling growth they did not, however, properly prevent soft rot of in vitro plants. Further studies on preventing soft rot of in vitro plants is needed.