Browsing by Author "Cantini, Elisabetta"

Background. Chronic diseases are characterised by an increase of lipopolysaccharide (LPS) in the systemic circulation, a marker of gut permeability and inflammation. The intake of red and processed meat (RPM) is associated with the development of chronic diseases. In Finland, men consume about 760 g/week of RPM, which is higher than the intake recommended by the EAT-Lancet’s planetary diet (maximum 196 g/week). Studies that investigate the effects of combining plant- and animal-based protein intake on gut health and immunity are limited. The aim of this thesis is to answer whether the partial replacement of RPM intake with legume-based products would prevent the translocation of LPS from the gut to the systemic circulation of healthy Finnish men. Methods. Altogether 102 participants were recruited in Finland for the Bean man study. They were stratified and randomly divided into two dietary groups for six-weeks intervention trial: the RPM group (Meat) and the RPM and bean group (Bean). The serum was collected and used to investigate the Toll-Like Receptor 4 (TLR4) activation by LPS in Human Embryonic Kidney Cells 293 carrying TLR4, by using the QUANTI-Blue™ colorimetric assay. Statistical analysis included t-test, ANCOVA and Pearson’s correlation analysis with the p value set at 0,05. Results. There were no differences in TLR4 activation between the intervention groups (p=0,59). Red meat and processed meat intakes were weakly correlated with TLR4 activation (p= 0,08 and p= 0,013 respectively), Body Mass Index and age were not correlated with TLR4 activation (p= 0.87 and p=0.81, respectively). Conclusions. Partially replacing RPM with legume-based products did not show a significant decrease in TLR4 activation in vitro among the bean group.