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Browsing by Author "Fabritius, Anita"

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  • Fabritius, Anita (2023)
    The study consists of two parts: examining the accumulation of americium and plutonium to human ribs, and the accumulation to murine osteoblasts. In the cellular studies stable europium was used as an actinide analogue. After removing the organic material from the bones, americium and plutonium were separated from the dissolved bones by using extraction chromatographic methods. DOWEX, U/TEVA, and TRU resins were used to separate plutonium and americium from impurities. The sample activities were measured using alpha spectrometry. On average, 23.03 mBq/kg of plutonium-238, 12.58 mBq/kg of plutonium-239,240 and 9.81 mBq/kg of americium-241 were measured in the bone samples. The activity concentrations are calculated using the wet weight of the bone. Murine preosteobasts (MC3T3-E1) were mineralized for 12 days. The mineral was subsequently collected for SEM/EDS analysis. Non-mineralized cell fractions were collected for a fractioning study, where the cell internalized europium, membrane bound europium and extracellular europium fractions were collected and measured with MP-AES and ICP-MS. The used europium concentrations were below minimum detectable activity of the MP-AES, but measuring the samples on the ICP-MS showed that the percentage of the internalized europium increases at higher europium concentrations in cell culture media. At 0.2 μM europium concentration, on average, 28.6% of the europium was inside the cells and 71.4% in the media, while at 2.0 μM europium concentration, 30.8% of the europium was inside the cells and 68.5% in the media.