Browsing by Subject "ICC"
Now showing items 1-2 of 2
-
Modeling early neuronal development in Kabuki Syndrome using human induced pluripotent stem cells. (2022)Neurodevelopmental disorders (NDDs) are disabilities in which the formation and development of the central nervous system is altered. NDDs severely impact the quality of life of the individuals that are affected by them, however little is known about the causes or the molecular mechanisms that are behind their onset. For this reason, being able to model them is pivotal to our society since, by understanding the mechanisms underlying such disorders, we could develop possible treatments. Previous research has suggested that disturbances in the early neuronal development could be at the basis of NDDs onset. Therefore, in this work, I have modeled neuronal differentiation in Kabuki syndrome (KS), a known NDD, assaying the expression of key early neurodevelopmental markers at four specific timepoints, using induced pluripotent stem cell (iPSC) technology. By concurrently differentiating three KS patient-derived and three control iPSC lines to neural precursor cells (NPCs) and profiling them with immunocytochemistry (ICC) and quantitative real-time PCR (RT-qPCR), I was able to identify differences in the early developmental trajectories of NPCs between the two conditions. The ICC data suggested that differentiating KS cell lines incur in precocious differentiation when compared to control cell lines, suggesting that the disease-causing mutations could lead to accelerated neuronal maturation of early NPCs. However, RT-qPCR analysis of the expression patterns of key neurogenesis markers was unable to statistically confirm the observed trend between the two phenotypes, likely due to limitations in statistical power. Despite this, the expression of four out of seven NPC markers was higher in early KS cells than in control cell lines, supporting the hypothesis of accelerated neuronal maturation. Taken together, this work highlighted some of the challenges related to iPSC-based disease modelling studies, and the need to further confirm the inferred mechanisms of asynchronous neuronal development observed in this work.
-
(2020)Sexual violence in conflict against men and boys has been prevalent throughout conflicts in history. Nonetheless, this form of violence has received less attention in international scholarly and judicial discourses than similar forms of abuse against women and girls. Building on critical feminist thought, this thesis seeks to study the thematic development of international criminal tribunals’ and courts’ discussions on sexual violence against men and boys in conflict. Further, it examines how feminist legal activists’ framings of conflict-related sexual violence are visible in international criminal law (ICL) case law and what consequences these framings have on male victims of sexual violence. Since the 1990s, the feminist debate on sexual violence in conflict produced different strategies of influencing the international debate on conflict-related sexual violence. Governance feminist have sought to do so from within ICL institutions and critical feminist have mostly reviewed and engaged in the discourse and its consequences largely as external actors. This thesis argues that in an attempt to include women’s concerns in ICL case law, governance feminism introduced a gender language to ICL that reduces a contextualising analysis of gender-based violence, which lies at the heart of feminist legal thought and advocacy, to a predominant focus on sexual violence. The adoption of this governance feminist understanding of gender has curtailed the recognition and documentation of conflict-related sexual violence against men and boys in ICL court and tribunal cases. The thesis shows that conflict-related sexual violence against men is primarily discussed by ICL benches under the themes of rape, torture, imprisonment and in some contexts as psychological violence. The examined courts utilise a simplistic understanding of conflict-related sexual violence. They frame male victims predominantly through the same lenses as female victimhood as feminised and humiliated individuals caught in power contestations, sex binaries with men as perpetrators and women as victims, and one-dimensional ethnic divisions. Sexually abused men who do not conform to heterosexual norms, are members of specific ethnic groups or have been violated in detention conditions are thus often not recognised as victims. Their evidence is frequently excluded from ICL court proceedings due to the absence of comprehensive deconstructions of social and cultural norms surrounding gender conceptions in ICL case law. The lack of this dismantling of gender constructions in ICL proceedings raises further questions on the treatment of complex identity questions in ICL courts and the role of critical feminist legal thought as either reform driven or critical lens on discussions of gender in ICL.
Now showing items 1-2 of 2