Browsing by Subject "biolasi"

Compared to many other countries, Salmonella prevalence in finnish poultry, bovines and pigs are at a low level. This low level has been obtained because of the national salmonella control programme. However, Salmonella still occur in feeds and at broiler farms in Finland and EU. Bioactive glass (Bioglass) could be a potential feed additive for preventing Salmonella in poultry since many new feed additives have got EU ac-ceptance. Bioactive glasses have been found bactericidic when used in dentistry and artificial tissue technology as implant material. The aim of this thesis was to study if and how bioglass effects Salmonella enterica bacteria in chicken feed and gut contents. Further, the purpose was to determine if bioglass can reduce the amount of Salmonella in the sample matrixes using the spread plate method. Six strains of Salmonella enter-ica from Hambi culture collection (HAMBI 1111, 1112, 1306, 1513, 2317 ja 2331) was added to the matrixes. Commercial XLD medium was used to determine the amount of Sal-monella in each time point of the test. 0 - 10 % of bioglass was tested for bactericidic effects. The ability of bioglass to inhibit the growth of Salmonella was attempted to demonstrate with transmission electron microscopy and using gram staining and light microscopy. The results indicate that the bactericidic effects of bioglass work in the sample matrix-es nearly as well as in liquids. The added amount of Salmonella was significantly re-duced with the higher concentrations of bioglass. Lower levels of bioglass did not show bactericidic effects or the inhibition of the growth of Salmonella was minor. The bacte-ricidic effect is thought to be based on the rise of pH and the adherence to bacterial cell wall. TEM imagines illustrated the attachment of the bioglass particles to degrad-ing cells of Salmonella Typhimurium (HAMBI 1112). Bioglass could be a potential feed additive for the prevention of Salmonella in broilers.