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Browsing by Subject "physiology"

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  • Functional characterisation of CETS genes in Fragaria vesca L. 
    Andrés Jiménez, Javier (2017)
    The Rosaceae family accounts for more than 90% of the total fresh fruit production in Finland and for more than 25% worldwide in 2013. Thus, improving the yield potential of Rosaceae species works in favor of economic growth and food security. To achieve that, expanding the knowledge on Rosaceae species is required. Fragaria vesca (F. vesca), particularly the everbearing F. vesca semperflorens accession ‘Hawaii-4’ (H4), provides several features that makes it formidable as a model organism for the study of physiological processes in Rosaceae species, where the CENTRORADIALIS/TERMINALFLOWER 1/SELF-PRUNING (CETS) genes play a remarkable role. In this Thesis, I have studied the expression patterns and functions of the CETS genes by performing gene expression analysis, generation of H4 transgenic lines and observation on H4 plants (both transgenic and wild type) grown under different sets of conditions. My results confirmed the expression patterns and functions previously reported for F.vesca TERMINAL FLOWER1 and F.vesca FLOWERING LOCUS T1. I showed that F.vesca CENTRORADIALIS-LIKE2 is a floral repressor and demonstrated that F.vesca FLOWERING LOCUS T3 is a flowering promoter. Additionally, our data suggest that F.vesca MOTHER OF FT is directly related with stolon formation and that F.vesca CENTRORADIALIS-LIKE1 is most likely a floral repressor. This new information could be used in the future to improve the efficiency of Rosaceae crops farming by adapting flowering and vegetative responses and also for breeding more productive Rosaceae crops.
  • Perunan Y-viruksen testaaminen korkeassa happi-hiilidioksidipitoisuudessa idätetyistä perunoista 
    Solarmo, Elina (2011)
    Potato virus Y (PVY) is currently the most yield and quality limiting pathogen of the cultivated potato (Solanum tuberosum L.) globally. While yield losses specific to PVY are hard to measure in presence of other pathogens they are estimated as 20 to 80 %. The most important way by which the virus spreads are virus-infected seed potatoes. High quality seed potato is very important for food, food industry and starch potato production. Visual inspection of the potato plants underestimates usually the real percentage of PVY infection. Laboratory tests provide more accurate results about the incidence of infections. The problem with testing PVY is that the virus cannot be detected reliably from samples taken from dormant tubers. Different treatments have been used to break dormancy of tubers e.g. chemicals (Rindite, bromoethane), plant hormones (gibberellic acid) and adjusted storage temperatures (cold and heat treatment). The results have varied a lot. In this thesis an experiment with oxygen-carbon dioxide (O2 40 %-CO2 20 %) treatment with different periods of time was used to end dormancy of potato tubers. The aim was to test whether the treatment could end dormancy of tubers earlier than normally and to see if the treatment has an effect on detection of PVY. One aim was also to test how reliably PVY could be detected from tuber and sprout samples compared with potato leaf samples which are normally used for virus testing. Results from the sprouting treatment were variable and could not be readily generalized. The treatment had no effect on the detection of PVY incidence. When the different plant materials were compared with each other, tuber material showed the lowest PVY percentage when compared to sprouts and leaves. Testing sprouts also underestimated the incidence PVY. The best material for testing PVY in potatoes were the leaf samples.

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