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Browsing by Subject "ubiquitin"

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  • Lindholm, Katariina Marianne (2015)
    Arabidopsis (Arabidopsis thaliana J. Thal) is a common model species in plant research. Although its genome is completely sequenced, the complete effects of some genes remain undetermined. The aim of this study was to investigate the role of several E3 Ubiquitin (Ub) ligases in Arabidopsis thaliana development through insertional mutant lines from the SALK collection. Sixteen mutant lines in a wild-type Columbia-0 (Col-0) background were subjected to genotyping, and the subsequent confirmed homozygous lines were analyzed for developmental phenotype. Greenhouse experiments were conducted on the wild type Columbia (Col) and sixteen mutant lines. Yield parameters such as days to bolting and silique surface area were studied to identify differences among the mutant lines relative to wild-type. After phenotyping, statistical analysis was performed to determine if there were any significant differences among the lines. The mean of both number of days to bolting and average silique surface area, among line 8, line 9, and line 13b plants, were shown by one way ANOVA to be significantly different than wild-type. These results suggest that those E3 ubiquitin ligases might be involved in the regulation of plant growth and development.
  • Nousiainen, Ruth (2023)
    Background: Hepatoblastoma (HB) is the most common malignant liver tumor among children. Methods: To gain new insight into the pathobiology of HB, we performed RNA sequence analysis on 5 patient-derived xenograft lines (HB-243, HB-279, HB-282, HB-284, HB-295) and 1 immortalized cell line (HUH6). Primary hepatocytes were used as control. Results: We found 2,868 genes that were differentially expressed in all of the HB lines on mRNA level. The most upregulated genes were ODAM, TRIM71, and IGDCC3, and the most downregulated were SAA1, SAA2, and NNMT. Protein-protein interaction analysis identified ubiquitination as a key pathway dysregulated in HB. UBE2C, encoding an E2 ubiquitin ligase often overexpressed in cancer cells, was markedly upregulated in 5 of the 6 HB cell lines. Validation studies confirmed UBE2C immunostaining in 20 of 25 HB tumor specimens versus 1 of 6 normal liver samples. The silencing of UBE2C in two HB cell models resulted in decreased cell viability. RNA sequencing analysis showed alterations in cell cycle regulation after UBE2C knockdown. UBE2C expression in HB correlated with inferior patient survival. Conclusions: We conclude that UBE2C may hold prognostic utility in HB and that the ubiquitin pathway is a potential therapeutic target in this tumor.