Browsing by Author "Mulari, Perttu"

Cancer is a significant challenge for human health, causing approximately 9,6 million deaths annually. Critical problem in cancer treatments is chemoresistance, where cancer cells develop a resistance to the drugs used in the treatment. Chemoresistance has been studied for example with fluorescence spectroscopy and mass spectrometry. However, there are better options, as for example autofluorescence and photobleaching are a challenge in fluorescence spectroscopy and sample destruction in mass spectrometry. Vibrational spectroscopy is a label-free global method, which offers data extensively. The aim of this study is to evaluate the applicability of vibrational spectroscopy for studying chemoresistance using cisplatin sensitive TYK-nu cells and cisplatin resistant TYK-nu-CPR cells. Additionally, it is studied if there are difference on the spectra of a sample measured in liquid and dried. Infrared and Raman spectroscopy, which are used, are non-invasive and label free techniques that provide information about the biochemical structure of the samples. Techniques used in this study are infrared spectroscopy, Fourier transform Raman spectroscopy and confocal Raman spectroscopy. All techniques successfully distinguished the cell lines from each other according to the principal component analysis. Ratiometric analysis suggests a higher lipid to protein ratio in TYK-nu-CPR cells, which might be the separating factor between TYK-nu and TYK-nu-CPR cells. Liquid seemed to cause overlapping with peaks originating from the cell, and a large water related peak, which has to be taken into account if measuring cells in liquid.