Browsing by Subject "fotoionisaatio ilmanpaineessa"

Miniaturizing of analytical techniques in mass spectrometry has received a lot of attention amongst scientists. The gains of miniaturization of analytical systems are rapid analyses, lower solvent consumption, the option for automatization and lower costs. A glass-made microchip heated nebulizer and a newer version, steel-made nebulizer, have been recently developed. The aim of this study was to evaluate and compare performances of the nebulizers. Changes in test conditions and effects of different dopants to intensiveness of the analytes' signals were analyzed. Speed of nebulizer gas, speed of analyte flow and magnitude of heating were the parameters of the changes in test conditions. The temperature of the flow from the nebulizers was also measured and analyzed. The intensiveness profiles of the analytes between the nebulizers were unequal, when changes in the speed of nebulizer gas and magnitude of heating were measured. The nebulizers reacted the same way to changes of the speed of analyte flow. The faster the analyte flow was, the more intensive the analytes' signals were. The steel tube nebulizer generated on average more intensive signals of the analytes than glass-made microchip. Temperature of the glass-made nebulizer was considerably higher than that of steel tube nebulizer. The most intensive signals of the analytes were achieved when toluene was used as a dopant. Steel tube nebulizer was more efficient in ionizing analytes than glass-made microchip. However, with steel tube nebulizer it could be difficult to analyze compounds with high boiling point. One goal of this study was to combine the steel tube nebulizer with capLC, but due to technical failures of the capLC equipment this was not possible. In the future, it would be beneficial to improve the steel tube nebulizer's heating mechanism. Also it could be combined with other ionization techniques as has been done with glass-made nebulizer.