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Integration of total chemical analysis with microchip capillary electrophoresis

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dc.date.accessioned 2013-08-06T13:43:42Z und
dc.date.accessioned 2017-10-24T12:19:38Z
dc.date.available 2013-08-06T13:43:42Z und
dc.date.available 2017-10-24T12:19:38Z
dc.date.issued 2013-08-06T13:43:42Z
dc.identifier.uri http://radr.hulib.helsinki.fi/handle/10138.1/3025 und
dc.identifier.uri http://hdl.handle.net/10138.1/3025
dc.title Integration of total chemical analysis with microchip capillary electrophoresis en
ethesis.discipline Analytical Chemistry en
ethesis.discipline Analyyttinen kemia fi
ethesis.discipline Analytisk kemi sv
ethesis.discipline.URI http://data.hulib.helsinki.fi/id/31006aab-9d8f-4c16-a0dc-b20067339529
ethesis.department.URI http://data.hulib.helsinki.fi/id/c2dd677c-da9c-4011-94b0-27b1585ac1cb
ethesis.department Kemiska institutionen sv
ethesis.department Department of Chemistry en
ethesis.department Kemian laitos fi
ethesis.faculty Matematisk-naturvetenskapliga fakulteten sv
ethesis.faculty Matemaattis-luonnontieteellinen tiedekunta fi
ethesis.faculty Faculty of Science en
ethesis.faculty.URI http://data.hulib.helsinki.fi/id/8d59209f-6614-4edd-9744-1ebdaf1d13ca
ethesis.university.URI http://data.hulib.helsinki.fi/id/50ae46d8-7ba9-4821-877c-c994c78b0d97
ethesis.university Helsingfors universitet sv
ethesis.university University of Helsinki en
ethesis.university Helsingin yliopisto fi
dct.creator Railila, Annika
dct.issued 2013
dct.language.ISO639-2 eng
dct.abstract Total chemical analysis systems (TAS) have been a hot topic during the last 10 years mainly due to the possibilities of analyzing small samples, as small as nanoliters and femtoliters, in a quick manner and requiring only small amounts of buffers. In this thesis the focus is on the studies carried out on microchips that integrate sample pretreatment , capillary electrophoretic (CE) separation and detection steps.. The techniques used have mainly been developed for the analysis of biological samples, such as drugs, proteins and biomarkers, and for DNA profiling. On-line sample pretreatments include preconcentration, sample cleanup and derivatisation, all with the aim to minimise sample losses and the effective analysis time. By preconcentrating the analytes on-line the detection limits can be decreased and the analyte detection responses increased and band broadening is avoided. In addition, TAS devices allow portable and in-situ analyses. making the systems useful especially in environmental studies, and in forensic and medical science. The experimental part of the thesis was focused on the physico-chemical characterization of some phosphonium-based ionic liquids. The specific aim was to get a further insight into phosphonium-based ionic liquids (ILs) used in electrokinetic chromatography (EKC) as psedostationary phases. The critical micelle concentration (CMC) values for the ILs were determined for the calculation of the distribution constantsfor the model analytes. Another purpose of the work was to investigate the ionicity of some phosphonium-based ILs that is of great importance of understanding the solvent properties and vapor pressures of the ILs. In order to calculate the ionicity, the temperature-dependent conductivity is needed along with the viscosity. Therefore the temperature-dependent conductivity of some ILs was studied. The CMCs determined by the CE method was 0.50 mM for [P14444]Cl and 0.68 mM for [P14444]OAc. The measurements were done in the same way in both cases and the electropherograms looked very similar. The water-soluble phosphonium-based ILs studied in this work seemed to have a linear temperature dependent conductivity when diluted with water. In contrast, the concentrated ILs had a much lower conductivity and the temperature dependency was of a polynomial form. en
dct.language en
ethesis.language.URI http://data.hulib.helsinki.fi/id/languages/eng
ethesis.language English en
ethesis.language englanti fi
ethesis.language engelska sv
ethesis.thesistype pro gradu-avhandlingar sv
ethesis.thesistype pro gradu -tutkielmat fi
ethesis.thesistype master's thesis en
ethesis.thesistype.URI http://data.hulib.helsinki.fi/id/thesistypes/mastersthesis
dct.identifier.urn URN:NBN:fi-fe2017112251902
dc.type.dcmitype Text

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