Browsing by discipline "Molekyylibiologia"

This study compared proliferation rate and expression of cardiac stem cell markers in one day seven days old mice hearts (1D and 7D). Primary antibodies Ki-67 and C-kit were used in immunohistochemistry and primers C-kit, GATA-4, Myh6, Sca-1 and Mesp1 were used in quantitative PCR. This study found a considerably higher proliferation rate and a higher number of C-kit+ stem cells in 1D than in 7D samples, but the difference was not statistically significant due to a small sample size. A significant difference in the expression of other studied markers was not found. These findings suggest that a decrease in proliferation rate and the number of C-kit+ stem cells coincide with the loss of regenerative potential. Therefore, it is possible that either, or both, of these changes are responsible for the closure of the regenerative window.