Browsing by Subject "Fv/Fm"
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(2021)To determine the role of metalloprotease EGY1 and hormone signalling pathways in PSII repair cycle, a mutant named white because of early senescence was identified having 4bp deletion in EGY1. To further characterize the growth responses in white mutant, two suppressors (white suppressor 1 and white suppressor 2), mutated in STAY GREEN1 (SGR1) which prevents chlorophyll degradation, restored the normal white phenotype was identified upon suppressor mutant screens. This study investigated the effect of chloroplast translation inhibitors (lincomycin/chloramphenicol) and MV (methyl viologen) on photosynthesis in Arabidopsis thaliana single and double white mutants. Furthermore, a second goal was to verify the correct identification of the mutations in white suppressor 1 and white suppressor 2. Western blotting and pulse amplitude modulated fluorimeter (PAM) was used to quantify the D1 protein (reaction core of PSII) levels and photochemical efficiency (Fv/Fm) respectively. Immunoblotting revealed a pronounced decrease in D1 levels for both white and egy1. PAM results showed a high tolerance of white mutant towards lincomycin/chloramphenicol. The white suppressors complemented the lincomycin/chloramphenicol tolerance of white mutant. The white mutant was highly MV sensitive. This MV response was altered in white double mutants (white ein2-1, white sr1-4D and white rcd1-4), suggesting that hormone signalling was involved in the response to MV. The decreased abundance of D1 in the white mutant suggests a role for EGY1 in PSII assembly and D1 turnover under light stress. In all assays (immunoblotting and PAM), the white mutant and egy1-2 gave the same results, this confirms the correct identification of the white mutant as a new egy1 allele. The successful restoration of lincomycin /chloramphenicol tolerance by white suppressors (S1 and S2), implicates that chlorophyll breakdown impacts on correct photosynthesis function. The suppressors S1 and S2 were transformed with wildtype SGR1, which restored the white mutant phenotype. Thus, the suppressor phenotype was caused by mutations in SGR1.
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(2023)Forests’ exposure to drought is increasing as a result of climate change. Drought increases tree mortality and the likelihood of wildfires. Mitigating damages contributed to by drought is important in order to secure access to ecosystem services. Remote sensing can be applied in drought detection, development monitoring, wildfire risk assessment, and phenotyping for resistance breeding. Hyperspectral imaging (HSI) combines spectral and spatial information, which may be used as a proxy to estimate biochemical and physiological traits of plants, including water content and response to water stress. More affordable and compact HSI cameras have become available in recent years, but their use in remote sensing of forests is still somewhat novel. The photochemical reflectance index (PRI) is an optical vegetation index, that was originally defined based on its diurnal response to the epoxidation state of xanthophyll cycle. PRI has been successfully used to capture drought stress and recovery on various scales on mainly broadleaf species. PRI responds to drought due to stomatal closure leading to downregulation of photosynthesis increasing the need for light energy dissipation. The aim of this thesis was to assess the feasibility of monitoring drought development and recovery of Pinus sylvestris seedlings in ambient greenhouse conditions using hyperspectral imaging. The hypotheses addressed in this thesis were: 1) there is a relationship between physiological variables and optical vegetation indices from HSI, and 2) PRI captures reversible photoprotective energy dissipation and responds to stress, outperforming the chlorophyll-responsive indices over the duration of the drought and recovery period. Imaging and additional measurements were performed on 8 4-year-old Pinus sylvestris seedlings. Pinus sylvestris, is the most common tree species in Finland, and it is widely utilized in the Finnish forestry industry. Half of the seedlings were exposed to 17 days of progressive drought and half were watered regularly. HSI and physiological variables were measured every few days for the duration of pre-drought, drought, and recovery periods. Physiological variables, which were leaf water potential and maximum quantum yield of PSII photochemistry (Fv/Fm), were measured to validate drought stress development. Additionally, meteorological conditions and soil moisture content were monitored. Non-imaging leaf level reflectance was measured on 3 days, which represented pre-drought, height of drought, and end of recovery. This thesis is concerned with the data collected on these three days. The results of this thesis showed that HSI based PRI between drought and control plants differentiated significantly during height of drought, and mostly recovered by the end of the scheduled recovery period. Chlorophyll-responsive red edge index responded to drought but did not show signs of recovery. Relationship between HSI based PRI and physiological variables Fv/Fm and leaf water potential was significant. These results demonstrate that HSI can be used to capture progressive drought stress development and recovery at a seedling canopy level in boreal evergreen saplings under greenhouse conditions.
Now showing items 1-2 of 2