Browsing by Subject "ITS2"

Genus Vicia comprises more than 190 species that chiefly grow in the temperate area of Europe and Asia as well as some parts of tropical Africa, North America, and South America. Based on inflorescence length and the presence of nectariferous spots on the stipules, the genus is divided into two subgenera Cracca (Vicilla) and Vicia, consist of 17 and 9 sections, respectively. The most widely known species is faba bean (V. faba), also referred to as poor man’s meat. However, the close relatedness of V. faba from section Faba with section Narbonensis is still uncertain while V. hirsuta placed in section Cracca although morphological and biochemical evidence support its separation from Cracca. Moreover, there is also the issue of further dividing the genus by separating Ervum as the third subgenus. Therefore, this work aimed to examine the evolutional relationship among 24 selected species of genus Vicia by using housekeeping genes, nuclear ribosomal internal transcribed spacer (ITS2) and mitochondrial cytochrome c oxidase subunit I (Cox1). The plant DNA was extracted and amplified with gene-specific primers followed by PCR product clean up by using GeneJET. The cleaned DNA fragments were cloned with a pGEM-T vector and sequenced. The sequence alignment was performed using CLUSTAL W and MAGA-X to construct maximum likelihood phylogenetic trees. The result showed that most of the species were monophyletic and there were few paraphyletic groupings. Species in section Narbonensis were in the last part of the tree whereas V. faba was placed in the upper part far from those species. V. hirsuta formed a clade with section Narbonensis rather than with section Cracca. Similarly, section Ervum grouped with species of section Cassubicae and Panduralae instead of being separated from the rest of the species. In conclusion, this work showed the molecular phylogenetic tree of 24 selected species of genus Vicia which are able to provide more acceptable details to classify as compared to previous Vicia morphological phylogenetic tree. ITS2 was a more informative tool than Cox1 for dealing with phylogenetic relationships.