Browsing by Subject "Exoelectrogenesis"

Renewable energy is the key for a sustainable future in a world currently run by coal and oil, and one of these sources could be bioelectrochemical systems [McCormick et. al., Energy Environ. Sci, 2015]. This is very different from traditional renewable energy sources, in that traditionally the process for generating the solar cells requires exotic material, or has a relatively extensive manufacturing process [Ren et. al., Solar Energy, 2020]. One type of these bioelectrochemical systems are biophotovoltaic systems, which utilize solar energy and water to produce electrons or other reducing agents outside of the organism, which can then be harvested for external usage [McCormick et. al., Energy Environ. Sci, 2015]. This type of system has many different focuses to improve efficiency, including substrate design, reactor design, and electrode properties [Anam et. al., Sustainable Energy Fuels, 2021]. While these are important, there is another avenue to be explored, namely the exoelectrogenesis pathway itself [Okedi et. al., bioRxiv, 2021]. This pathway analysis has been explored briefly with Hilbert-Huang transforms to figure out their oscillatory components, which has been partially mapped to photosystem II core expression [Okedi et. al., bioRxiv, 2021]. In my analysis, I will be using generated data from cyanobacteria which exhibit enhanced photosystem II and see if the exact mechanisms for this phenomenon can be captured. The data provided by the sequencing vendor comes in a FASTA Extension format, so the process and tools to translate this data into usable variant calling format files will be described. I will then iterate the additional analysis in the way of variant comparisons through strain concordance with gene comparisons, as well as phylogenetic trees. The first analysis is to compare a wild type to a mutated strain, with subsequent analysis being to compare multiple wild type strains to each other. Further analysis on phenotype expression compared to the variant calling will also be explored.