Skip to main content
Login | Suomeksi | På svenska | In English

Browsing by Subject "Diet"

Sort by: Order: Results:

  • Mass Spectrometry-Based Lipidomics Indicates that Consumption of Fatty Fish Alters the lipid species Profile of Human LDL 
    Bhalke, Monika (2020)
    Lipoproteins are biochemical carriers of the insoluble lipids. They are complexes combining lipids and proteins for the transport of lipids. Amongst the type of lipoproteins are low-density lipoproteins (LDL) which are prevalent in various diseases such as obesity, diabetes, atherosclerosis, and other cardiovascular diseases (CVD). Omega-3 fatty acids are polyunsaturated fatty acids (PUFA) that are essential components of lipid metabolism and play a significant role in the human diet. Omega-3 PUFAs such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are derived from fish and are necessary for proper cardiovascular functioning. Because the human body is unable to produce enough quantities of some omega-3, diet is an important source for its availability. When a diet is rich in saturated fats, the above-mentioned diseases transpire. This study investigated how consumption of two fish diets, Lean fish and Fatty fish, influence the lipid species of human LDL particles. The lipid species analysed in this study are phospholipids such as phosphatidylcholine (PC), sphingomyelin (SM), and lysophosphatidylcholine (LPC), and cholesteryl esters (CE), and triacylglycerols (TAG). A total of 42 volunteers with a history of impaired fasting glucose had randomly been divided into two groups: fatty fish (4 fish meals/week) and lean fish (4 fish meals/week) for 12 weeks. Blood samples had been collected from the volunteers before and after consumption of the fish meals and LDL particles had been isolated from the blood samples by ultracentrifugation. In this study, the lipids were extracted by Folch method, and the extracted lipids were analysed using Triple quadrupole mass spectrometry. The lipid class profile did not change due to the two fish type diets. However, the consumption of fatty fish diet increased the levels of lipid species of PC, LPC, and CE containing EPA and DHA acyl chains, while decreasing levels of several TAG species. Lean fish induced minor changes in the lipid composition of LDL particles. Based on these results, fatty fish diet alters the plasma LDL lipidome profile with changes induced to both the surface and the core composition of the LDL particles in a positive way regarding cardiovascular health.
  • Year-round variation of total mercury in female and male European perch (Perca fluviatilis) muscle, liver and gonad tissues in a boreal humic lake. 
    Deb, Aruna Rani (2024)
    Since mercury (Hg) may biomagnify in food webs and bioaccumulate in living things, it is considered a dangerous element globally. The two most toxic forms of mercury are methyl mercury (MgHg) and dimethyl mercury (DMgHg). The dietary Hg consumed by fish is mostly removed through the intestine, but some of the MeHg bioaccumulates and is delivered to various organs, such as the liver, kidney, muscle, or gonad. The perch (Perca fluviatilis), the national fish of Finland serves as both a popular food fish and a monitoring species for assessing the chemical health of lakes. Fish tissue exhibits seasonal variations in mercury levels, which are thought to be produced by growth dilution in the summer, which is related to rapid somatic growth during the growing season, and hunger in the winter, which condenses mercury in the muscle as well as during spawning since gonad development requires significant energy expenditure. There has been a considerable study on Hg concentration in fish but currently lack knowledge regarding potential seasonal variation in patterns of Hg content and bioaccumulation to understand the dynamics of Hg content and bioaccumulation. This study investigates (Q1): Does mercury content change in muscle, liver and gonad tissues of males and females of perch over the four seasons? (Q2): How does mercury bioaccumulation change seasonally in different organs between male and female perch? (Q3): How does the mercury content relationship among different organs (muscle, liver, and gonads) vary seasonally and between sexes? Materials were collected monthly from Lake Pääjärvi from April 2020 to March 2021, and categorized into four seasons: winter (January -March), spring (April-Jun), summer (July-September), and autumn (October-December). Each fish was taken of its length, weight, sex, and other tissues too. Total mercury (Hg) concentrations were determined in three organs (muscle, liver and gonad) in male and female perch separately. Using analysis of variance, the annual length-corrected THg content variation and simple linear regression analysis were used to examine the annual THg bioaccumulation variation and to test the relationships in Hg concentrations among three tissues separately in males and females. Seasonal THg levels in female perch significantly varied in muscle and gonads, not in the liver. Females had consistently higher THg in muscle and liver, while males had higher levels in gonads throughout the season. THg bioaccumulation peaked in spring and winter for both sexes in muscle, but lowest in autumn. Liver THg slope was highest in early summer for females and lowest in autumn. Gonads showed the highest slopes in summer for both sexes. The highest slopes between muscle and liver THg for females were in summer and for males in spring. Similar patterns were seen in both muscle-gonad and liver-gonad THg relationships. Female perch showed significant differences only in summer, while in males, the highest slopes were in autumn and lowest in summer. Long-term monitoring is crucial to understanding THg variation in fish.

Yhteystiedot

HELSINGIN YLIOPISTO