Browsing by Subject "dopamine"

Background: ProSAAS is a neuroendocrine peptide precursor implicated in various physiological pathways and several disorders. Despite its potential significance, there is a notable lack of studies exploring the roles of proSAAS and its derived peptides. Objectives: This hybrid systematic review aims to provide an overview of the neuroprotective role of proSAAS in brain-related disorders and its potential as a biomarker. The hypothesis is that the loss of proSAAS, known for its many neuroprotective properties, would affect dopaminergic and serotonergic neurons in zebrafish brains. Methods: Following PRISMA 2020 guidelines, this review includes studies on proSAAS in brain-related disorders and its biomarker potential, excluding non-brain-related physiological aspects. The focus is on dopaminergic and serotonergic systems in zebrafish. Searches were conducted on PubMed using keywords like "proSAAS," "aminergic system in zebrafish," "dopaminergic neurons in zebrafish," and "serotonergic neurons in zebrafish" on 04.05.2024, 09.05.2024, 11.05.2024, and 12.05.2024. Risk of bias was evaluated using the Cochrane Collaboration’s and AMSTAR 2 tools. For the experimental part, immunohistochemical analysis was conducted on zebrafish aged 4, 5, and 6 days post-fertilization. Results: A total of 103 studies were included in the systematic literature review. Six studies highlighted the neuroprotective role of proSAAS in neurodegenerative diseases. Two studies linked proSAAS to homeostatic upscaling, and five identified it as a potential biomarker for neurological conditions. Furthermore, nine studies investigated the role of proSAAS-derived peptides. Experimental results from immunohistochemical analysis showed no significant changes in dopaminergic and serotonergic systems between wild type, heterozygote, and knockout zebrafish. Discussion: Limitations include potential bias from included studies, small sample sizes and limited repetitions of the experiments. The review suggests proSAAS is critical for brain function and neurological conditions, though experimental findings did not show significant effects on dopaminergic and serotonergic neurons in zebrafish.