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Design embraces several disciplines dedicated to the production of artifacts and services. These disciplines are quite independent and only recently has psychological interest focused on them. Nowadays, the psychological theories of design, also called design cognition literature, describe the design process from the information processing viewpoint. These models co-exist with the normative standards of how designs should be crafted. In many places there are concrete discrepancies between these two in a way that resembles the differences between the actual and ideal decision-making. This study aimed to explore the possible difference related to problem decomposition. Decomposition is a standard component of human problem-solving models and is also included in the normative models of design. The idea of decomposition is to focus on a single aspect of the problem at a time. Despite its significance, the nature of decomposition in conceptual design is poorly understood and has only been preliminary investigated. This study addressed the status of decomposition in conceptual design of products using protocol analysis. Previous empirical investigations have argued that there are implicit and explicit decomposition, but have not provided a theoretical basis for these two. Therefore, the current research began by reviewing the problem solving and design literature and then composing a cognitive model of the solution search of conceptual design. The result is a synthetic view which describes recognition and decomposition as the basic schemata for conceptual design. A psychological experiment was conducted to explore decomposition. In the test, sixteen (N=16) senior students of mechanical engineering created concepts for two alternative tasks. The concurrent think-aloud method and protocol analysis were used to study decomposition. The results showed that despite the emphasis on decomposition in the formal education, only few designers (N=3) used decomposition explicitly and spontaneously in the presented tasks, although the designers in general applied a top-down control strategy. Instead, inferring from the use of structured strategies, the designers always relied on implicit decomposition. These results confirm the initial observations found in the literature, but they also suggest that decomposition should be investigated further. In the future, the benefits and possibilities of explicit decomposition should be considered along with the cognitive mechanisms behind decomposition. After that, the current results could be reinterpreted.

An experiment-driven approach to software product and service development is getting increasing attention as a way to channel limited resources to the efficient creation of customer value. In this approach, software functionalities are developed incrementally and validated in continuous experiments with stakeholders such as customers and users. The experiments provide factual feedback for guiding subsequent development. Although case studies on experimentation conventions in the industry exist, an understanding of the state of the practice is incomplete. Furthermore, the obstacles and success factors of continuous experimentation have been little discussed. To these ends, an interview-based qualitative survey was conducted, exploring the experimentation experiences of ten software development companies. The study found that although the principles of continuous experimentation resonated with industry practitioners, the state of the practice was not mature. In particular, experimentation was rarely systematic and continuous. Key challenges related to changing organizational culture, accelerating development cycle speed, measuring customer value and product success, and securing resources. Success factors included an empowered organizational culture and deep customer and domain knowledge. There was also a good availability of technical tools and competence to support experimentation.

While the role of IT and computer science in the society is on the rise, interest in computer science education is also on the rise. Research covering study success and study paths is important for understanding both student needs and developing the educational programmes further. Using a data set covering student records from 2010 to 2020, this thesis aims to find key insights and base research in the topic of computer science study success and study paths in the University of Helsinki. Using novel visualizations and descriptive statistics this thesis builds a picture of the evolution of study paths and student success during a 10-year timeframe, providing much needed contextual information to be used as inspiration for future focused research into the phenomena discovered. The visualizations combined with statistical results show that certain student groups seem to have better study success and that there are differences in the study paths chosen by the student groups. It is also shown that the graduation rates from the Bachelor’s Programme in Computer Science are generally low, with some student groups showing higher than average graduation rates. Time from admission to graduation is longer than suggested and the sample study paths provided by the university are not generally followed, leading to the conclusion that the programme structure would need some assessment to better incorporate students with diverse academic backgrounds and differing personal study plans.

Multiple myeloma (MM) is a malignancy of antibody-secreting plasma cells in the bone marrow (BM). MM is the second most common hematological malignancy, accounting for about 14% of blood cancers. Despite the improvements in the treatment of MM, the disease remains incurable, and essentially all patients end up relapsing. Deletion of chromosome 16q, the location of tumor suppressor CYLD, occurs in 35% of MM patients. CYLD is a deubiquitinase, most recognized for its function as a negative regulator of the nuclear factor kappa B (NF-κB) pathway. The loss of CYLD is associated with disease progression and worse survival in MM, but its significance in drug response is unknown. As the loss of CYLD is common in MM, determining its effect on drug response is essential. Analysis of data gained from MM patient samples were used to study the effect of CYLD copy number status on drug response. The treatments were selected based on previous research performed by our group. The effect of homozygous deletion was most significant in inducing resistance to BMS-754807, an inhibitor of insulin-like growth factor 1 receptor. To investigate the role of loss of CYLD on drug response in vitro, cell lines with CYLD knockout (KO) were created using the clustered regularly interspaced short palindromic repeats (CRISPR) – CRISPR associated protein 9 (Cas9) technology. An established CYLD-KO cell line was treated with carfilzomib, bortezomib, dexamethasone and BMS-754807 to assess the effect of CYLD-KO. The CYLD-KO slightly increased the sensitivity to BMS-754807, but essentially no differences were detected in response to the drugs. The effect of CYLD-KO was additionally explored to NF-κB - and Wnt-pathway activation by Western blot analysis, but due to technical difficulties, the results were inconclusive. The loss of CYLD is a common genetic aberration in MM, giving a survival benefit for the malignant cells. Based on the results from patient data analysis, the loss of CYLD could promote drug resistance to BMS-754807, and the effect should be further studied with more cell lines with CYLD-KO. As the population ages, and as the median age of newly diagnosed patients is 70, the need for efficient MM therapies increases. Studying the mechanisms behind drug resistance and sensitivity is essential in the aim of improving the efficacy of MM therapy and, in the end, the overall survival of the patients.

The use of fossil fuels is a significant contributor to greenhouse gas emissions, making the transition to zero-carbon energy systems and the improvements in energy efficiency important in climate change mitigation. The energy transition also puts the citizen in a more influential position and changes the traditional dynamics between energy producers and consumers when citizens produce their own energy. Furthermore, due to the opportunity and capacity of the demand side, the energy transition places a greater emphasis on energy planning at the local level, which requires new solutions and changes in the management of the energy system. One rising phenomenon is the potential of bottom-up developments like energy communities. Within the building sector, housing cooperatives have started to emerge as energy communities, offering a way to address the energy transition through bottom-up-driven energy actions that provide renewable energy and other benefits to the local community. This master thesis examines housing cooperatives' energy communities and their role in the energy transition. The research addresses the shared renovation project in Hepokulta, Turku, seen from the energy community perspective. Furthermore, the research highlights the importance of niche development in sustainable transition, acknowledging energy communities as socio-technical niches where development is partly embedded in renewable energy technology and partly in new practices. The concept of energy community is therefore analysed through the lens of Strategic Niche Management, which focuses on expectations, networks, and learning. This research aims to analyse how residents in Hepokulta perceive the energy community project through the niche processes and how the development of energy communities might affect urban development. Analysing the residents' perceptions provide insight into the energy community characteristics and the relationship between residents and the project development processes. Additionally, the analysis identifies matters that could be changed to improve the development. The thesis is a mixed methods research combining quantitative and qualitative data, which was collected through a survey sent to the eight housing cooperatives in Hepokulta. The research showed that residents perceive the shared project in Hepokulta as essential for the area's development. Moreover, many residents overlooked the social aspects of the development, highlighting the absence of the energy community perspective in the renovation. The findings suggest some weaknesses within the three niche processes, including the early involvement of residents and communication. Furthermore, although the residents perceived themselves as important actors and the literature emphasised the importance of the demand side in future energy systems, the research revealed that the connection between project development and the residents is still lacking. However, the analysis indicates that introducing additional actors could help the energy community develop. External assistance could, for instance, benefit the housing cooperatives by facilitating improvements in the decision-making processes, the network between actors, and the sharing of information and skills.

This master’s thesis includes two sections: the present summarizing report and the article manuscript. The purpose of the study is to explore the benefits of an acceptance and commitment therapy (ACT)-based intervention course to university students’ well-being, using both quantitative and qualitative methods. The COVID-19 pandemic has decreased university students’ well-being and increased their risk of burnout. The declined state of student well-being calls for measures in exploring ways of promoting student well-being and preventing study-related burnout. The intervention course aimed to increase students’ psychological flexibility and organized studying skills, as means to positively impact their well-being and studying. A total of 189 participated in the study in the spring of 2021. Students’ risk of burnout was measured pre- and post-intervention, to statistically define whether students experienced that the course impacted their risk of burnout. To explore the course benefits in depth, students’ written reflective reports post-intervention were analyzed using abductive content analysis. The results were statistically compared according to the risk of burnout group assigned pre-intervention. The results showed that the intervention course led to a positive change in students' risk of burnout. The content analysis revealed seven main categories of benefits of the course: 1. improved general well-being, 2. improved self-knowledge, 3. increased psychological flexibility, 4. improved study practices and study motivation, 5. increased self-compassion, 6. gained peer-support, and 7. improved organized studying. These benefits were experienced by students regardless of their initial risk of burnout. The study highlights the potential of ACT-based intervention courses in improving the well-being and studying skills of university students, suggesting a need for further research on burnout prevention through such measures. The prospective publication channel for the research article manuscript is Research in Higher Education by Springer.

Mucosal associated invariant T (MAIT) cells are a fairly recently described population of innate-like T cells. In humans, MAIT cells represent an exceptionally abundant population in the blood, where they account for 1–10% of all T cells. However, compared to conventional T cells, which can display an almost unlimited T cell receptor (TCR) repertoire, the specificities of MAIT cell TCRs are limited. This evolutionarily conserved subset displays a semi-invariant TCR which recognises riboflavin metabolites, produced by a wide range of bacteria and fungi, and presented on the major histocompatibility complex (MHC)-class I related (MR1) molecule. The function and significance of MAIT cells in health and disease have only started to be unravelled, and it is becoming increasingly clear that MAIT cells are also modulated in non-microbial diseases. Interestingly, MAIT cells have been shown to exhibit a relatively high expression of the transcription factor, Helios, compared to most other T cell subsets. The function of this translational activator and repressor, encoded by the gene IKZF2, in the MAIT cell population remains obscure. This study focused on the functional role Helios may play in regulating the activation of MAIT cells. Thus, by using siRNA to silence Helios expression, and flow cytometry to analyse any potential alterations in MAIT cell activation markers, we aimed to be able to characterise the kinetics and functional role of Helios in peripheral MAIT cells of healthy individuals. Here, we clearly established a striking upregulation of Helios in MAIT cells following 24 hours of stimulation. Moreover, we were able to achieve a >50% knockdown of Helios at the protein level, in this subset of T cells. Nevertheless, no significant difference in any of the activation markers we investigated was present between the MAIT cells with reduced Helios expression and their controls. This could, however, of occurred as a result of the toxicity that the transfection had on the functionally of the T cells. From these results, it is difficult to conclude any clear role for Helios in the activation of MAIT cells, and consequently, further research needs to be performed before any clear conclusions can be drawn.

The design of instructional material affects learning from it. Abstraction, or limiting details and presenting difficult concepts by linking them with familiar objects, can limit the burden to the working memory and make learning easier. The presence of visualizations and the level to which students can interact with them and modify them also referred to as engagement, can promote information processing. This thesis presents the results of a study using a 2x3 experimental design with abstraction level (high abstraction, low abstraction) and engagement level (no viewing, viewing, presenting) as the factors. The study consisted of two experiments with different topics: hash tables and multidimensional arrays. We analyzed the effect of these factors on instructional efficiency and learning gain, accounting for prior knowledge, and prior cognitive load. We observed that high abstraction conditions limited study cognitive load for all participants, but were particularly beneficial for participants with some prior knowledge on the topic they studied. We also observed that higher engagement levels benefit participants with no prior knowledge on the topic they studied, but not necessarily participants with some prior knowledge. Low cognitive load in the pre-test phase makes studying easier regardless of the instructional material, as does knowledge on the topic being studied. Our results indicate that the abstractions and engagement with learning materials need to be designed with the students and their knowledge levels in mind. However, further research is needed to assess the components in different abstraction levels that affect learning outcomes and why and how cognitive load in the pre-test phase affects cognitive load throughout studying and testing.

Many European citizens have growing concerns over climate change. This seems to go together with the debate about the impact of consumers’ personal dietary choices on climate change. Novel food protein sources are entering the European food market to replace or compensate meat protein sources. One protein food alternative are insect-based proteins. However, there is limited research as to how choices of alternative insect-based protein products may influence consumers’ carbon footprints. This study explores the potential of insect protein to reduce the carbon footprint associated with European food consumption. Three scenarios were formed to identify and describe options for reducing current levels of carbon footprints associated with the consumption of conventionally produced chicken meat. In the scenarios, soybean meal-based feeds used in conventional chicken production are replaced with insect-based feeds, and chicken products are replaced with protein food products from insects. Further, two different insect feeding sources are considered and compared to each other. A number of existing global warming potential values from a variety of Life Cycle Assessment studies focusing on chicken and insect production were collected to create a database for use in the scenario analyses. The database was utilised to assess the global warming impact of producing alternative insect protein on the carbon footprint of European food consumption. The results from the three scenarios indicate that the carbon footprint of food consumption can be reduced by replacing conventionally produced chicken meat with insect-based protein food products. However, insect-based protein products would have a positive impact on the carbon footprint only if the insects that are produced for use in feed or food are farmed with low-value side streams. Currently, the shift to an increased use of side streams in insect-based food production faces regulatory challenges in Europe. In the light of European efforts to encourage sustainable food alternatives, and considering the environmental benefits insects could offer as alternative proteins over options of conventional protein sources, there is a need for continued research on the environmental sustainability of insect eating and insect feeding, as well as the safety and regulatory issues related to the use of insect protein in food consumption.

Fibropapillomatoosi (FP) on maailmanlaajuisesti tunnettu merikilpikonnien kasvainsairaus. Se aiheuttaa merikilpikonnille solukuvaltaan hyvänlaatuisia sidekudoskasvaimia, jotka voivat johtaa normaalien elintoimintojen häiriintymiseen. Taudin syntyyn on aiemmissa tutkimuksissa luotettavasti yhdistetty alfaherpesviruksiin kuuluva Chelonid Herpesvirus 5 (CHHV-5), mutta taudin tarkkaa syntymekanismia ei tunneta, ja sen ilmentyminen vaatii myös muita, toistaiseksi tuntemattomia tekijöitä. CHHV-5:n uskotaan säilyvän merikilpikonnien elimistössä latentisti muiden herpesvirusten tapaan, ja sitä on havaittu useissa terveissä kudoksissa sekä FP:tä sairastavilla että terveillä eläimillä. CHHV-5:n tiedetään leviävän ainakin kasvaimista irtoavien infektoituneiden solujen kautta, mutta vasta-ainetasojen ja viruksen esiintyvyyden kartoitukset viittaisivat siihen, että on olemassa myös muita, toistaiseksi tuntemattomia tartuntatapoja. Aiemmissa tutkimuksissa liemikilpikonnien (Chelonia mydas) munuaisissa ja virtsassa on havaittu CHHV-5 DNA:ta, mikä voisi viitata viruseritykseen munuaisten kautta virtsaan. FP:tä havaitaan erityisesti nuorilla liemikilpikonnilla niiden kerääntyessä rannikkoalueille. Yhdysvalloissa Atlantin kaakkoisrannikolla samalla eläinryhmällä on havaittu rutiininomaisissa raadonavauksissa myös runsaasti alkuperältään tuntematonta munuaisytimen soluvälien sidekudostumista. Tämän tutkimuksen tavoitteena oli 1) kuvailla nämä sidekudostumismuutokset yhdistelemällä tautia ilmentäviltä eläimiltä kerättyä patologista, histopatologista ja epidemiologista tietoa, sekä 2) selvittää, voisivatko munuaisten sidekudostumismuutokset olla CHHV-5-tartuntaan liittyvä, aiemmin tunnistamaton kohde-elinvaurio. Opinnäytetyön kirjallisen osuuden on ohjannut Niina Airas ja johtanut Antti Sukura Helsingin yliopistossa. Tutkimuksen kokeelliset osuudet (qPCR-analyysi ja tilastollinen analyysi) on toteutettu Florida Atlantic Universityssä Harbor Branch Oceanographic Institutessa Annie Page-Karjianin johtamassa tutkimusryhmässä hänen ohjauksessaan. Työssä esitetyt histopatologiset tutkimukset on tehnyt Brian Stacy University of Floridassa. Floridan alueella kuolleen tai lopetetun nuoren liemikilpikonnan (62 yksilöä) munuaisten CHHV-5 DNA-kopiomäärät tutkittiin qPCR-menetelmällä, ja munuaisten sidekudostumismuutosten voimakkuus arvioitiin histopatologisesti asteikolla 1-3 (1 – lievä, 2 – kohtalainen, 3 – voimakas) kaksoissokeutetussa tutkimuksessa. Tulostemme perusteella sidekudostumismuutosten voimakkuus ei näyttäisi olevan sidoksissa liemikilpikonnien sukupuoleen, rantautumisaikaan tai kuntoluokkaan. Histopatologisiin muutoksiin ei liittynyt tulehdussolukertymiä tai havaittuja solunsisäisiä taudinaiheuttajia. Kaikilla suuremmilla kilpikonnilla muutokset olivat kohtalaisia tai voimakkaita, kun taas nuoremmilla lieviä tai kohtalaisia, mutta nämä erot eivät olleet tilastollisesti merkitseviä (Kruskal-Wallis, p=0,3). Vaikka 94%:lla (31/33) tutkituista eläimistä ei näkynyt lainkaan silminnähtäviä merkkejä sidekudostumisesta, mikroskooppisesti kohtalaista tai voimakasta munuaisten sidekudostumista havaittiin 90%lla (56/62) eläimistä. Kymmenellä (16%, 10/62) tutkituista eläimistä oli todettu FP, mutta vain viiden (8%, 5/62) eläimen munuaisessa havaittiin CHHV-5-DNA:ta. Näistä eläimistä neljällä oli fibropapillomatoosikasvaimia muualla kuin munuaisissa. Tulosten perusteella CHHV-5 ei vaikuttaisi olevan merkittävä etiologinen tekijä nuorten villien liemikilpikonnien munuaisytimen fibroosimuutoksissa Floridan alueella.