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Bartonella species are facultative intracellular bacteria causing variety of diseases in humans and also infects endothelial cells and erythrocytes. Some Bartonella species utilize VirB/VirD4-type IV secretion system (T4SS) in order to secret Bartonella effector protein A (BepA) which infects endothelial host cells by inhibiting the apoptosis. But the enterotoxin homolog in Bartonella gene A (EhbA) and the enterotoxin homolog in Bartonella gene B (EhbB) are found in the non-BepA Bartonella strains. In my Master’s thesis, I study the host cell binding activity and identify host cell surface receptor of EhbB in Bartonella. In my thesis, the cell adhesion of multimeric B proteins of enterotoxin homologue in Bartonella (Ehb) have been analyzed with cell adhesion assay using HEK293T, HeLa 229, Ea.hy926, and CHO-K1 cells. The assay was conducted with EhbB1 and EhbB 1-1C proteins from Bartonella Bovis strain Bermond and Bartonella strain spp 1-1C and the experiment indicated the cell adhesion activity of both EhbB proteins compared to the controls used in the experiment. Moreover, the binding activity of EhbB1 with Ea.hy926 was studied at several incubation time points, such as; 30 min, 2 hours, 4 hours, 6 hours, and 8 hours. Several incubation period of EhbB1 and EhbB 1-1C with Ea.hy926 cells did not enhance cell surface adhesion because the same absorbance compared to controls. The interaction of EhbB1 with cell membrane HEK293T was studied by using western blot on cell membrane preparation from Ea.hy926 cells which was used to identify possible protein receptor of EhbB1. The experiment suggests that EbB1 is binding to receptors present on the cell membrane of HEK293T which could be protein. The cell adhesion activity of HEK293T cell membrane with EhbB1 was analyzed by inhibition assay. This experiment indicated that EhbB1 protein attached to cell surface receptors present on the HEK29T cell membrane, which inhibited EhbB1 protein to attach to Ea.hy926 cells. This also indicate that the cell surface receptor for EhbB1 could be protein but requires further study.

Twisted-winged parasitoids (Insecta: Strepsiptera) are parasites of other insects, which are characterized by complex life cycles and extreme sexual dimorphism. They have three endoparasitic larval stages. In most Strepsiptera only the motile first instar larvae and adult males are free living whereas the adult females are endoparasites during their entire life. The winged males slightly resemble flies, while females are neotenic. Around 630 extant Strepsiptera species are known but this is probably an underestimation since many species are cryptic. The genus Stylops, parasitoids of mining bees of genus Andrena, is one of the biggest genera of Strepsiptera. Until recently it was thought that only one species, Stylops melittae, occurs in Europe. Studies using DNA-methods revealed that it is in fact a species complex of 30 species. This study investigated which of these species occur in Finland. In addition, it was examined if the female morphology of the species could be used in species identification, since males are rarely found. Andrena host specimens were examined from three major entomological collections and the collecting data of the observed Stylops was recorded to a database. Suitable specimens were DNA barcoded and the acquired DNA barcodes were compered for the ones in databases (BOLD, GenBank). The females were separated from their hosts and photographed for the morphological examination using different imaging methods (focus stacking, SEM). Based on this study there are five species of Stylops in Finland instead of one as previously thought: S. thwaitesi, S. ater, S. japonicus, S. nevinsoni and S. spreta. In addition, there is one species, which is most likely undescribed. All females of these species can be identified based on their morphology. The results include summaries of each species containing information about their morphology, ecology, distribution and abundance. This study gives valuable new information about the twisted-winged parasitoids in Finland. The occurrence of the genus Stylops in Finland based on current taxonomy was examined for the first time. The study gave novel information about the distributions of Stylops-species. Deviating from previous information Stylops japonicus is also found in Europe. Furthermore, the study revealed new information about the host-parasite relationships of the species. Many of the Andrena host species had not been associated with certain Stylops species before. There has not been research focusing on Strepsiptera in Finland for a long time. The material collected for this study works as a good foundation for the possible future studies in Finland. The results were used in the 2019 Red List assessment and two of the Stylops species were in the Red List of threatened species.

This thesis aims to find out what are the challenges in the implementation of corporate social responsibility (CSR) strategy in a large labour-intensive company in the service business. Companies have an essential role in sustainable development and they are seen as important actors in spreading awareness of sustainability (Simas et al., 2013). While CSR is a concept that is playing an important part in the modern business world and has been involved in companies’ strategies for years now, there is still a gap between the CSR strategy formulation and implementation (Engert & Baumgartner, 2016). The research presented in this thesis is based on a literature review of CSR strategy implementation and on a single case study in a service business company, SOL Group, with high employee turnover, low wage levels and physical and intense working conditions. The objective of this thesis is to identify potential challenges in the CSR strategy implementation in large companies in service business, and based on the findings, structure hypotheses for further research. The case study data collection included four group interviews with several participants in each, as well as direct observation in the field, and it is supported by documents from the case company and literature. From the literature review, two models were identified: the five levers model by Lacy et al. (2009) and the success factor model by Engert & Baumgartner (2016). These two models were used as theoretical frameworks for analysing the challenges in the successful implementation of CSR strategy in the case company. The main finding was that the major challenge in the successful implementation of CSR strategy is to commit and engage the employees to the strategy implementation. This thesis proposes hypotheses for the future, to continue this research as an attempt to bridge the gap between CSR strategy formulation and implementation. It is hoped that the insight from this thesis helps companies to identify their challenges in the CSR strategy implementation and provides some guidelines on how to proceed if wanting to achieve a full implementation of CSR strategy.

The purpose of this master's thesis is to study nature values within the Finnish national Natura 2000 network on state owned land. The six goals of this work were achieved: 1) Areas with most nature values were identified by prioritizing habitats of Natura 2000 directive (92/43/EU) within. Areas with high nature value were usually in very natural state and had good connectivity to other similar places, or they were spots of some very rare nature types. 2) It was found out that data used was suitable for identifying conservation values, 3) find out the suitability of Zonation software in conservation area management and maintenance planning and 4) find out how results will change if conservation status is taken into account. As an addition to these 5) the most considerable areas with high conservation value were identified and 6) "Zonation software in a nutshell" was produced in Finnish to assist Finnish state officials to use the software for conservation purposes. These results will help Metsähallitus (The Finnish Forest and Park Service) - Natural Heritage Service - to target resourcing of habitat management and restoration in and around the areas with most considerable nature values. It is essential to sustain these areas and their values so that their nationwide importance can be maintained into the future. Data used in this study covered areas that were classified as Natura 2000 habitats according to European Union Council Directive 92/43/EEC. Analyses were done by using Zonation software, a tool for spatial conservation prioritization. Data consisted of 68 Natura 2000 habitat types and their state of naturalness and representativeness. Zonation took into account the rarity, quality, importance, threat status, biodiversity value, congruity and connectivity of these habitat types. As a result software produces a map of conservation priorities and associated quantitative information, which facilitate identification of areas with most considerable nature values. These were identified both ocularly and with Zonation software. Analyses were done at two levels: all habitat types together and in subgroups following division to major habitat types, such as coastal environments, inland waters, meadows, alpine habitats, peat lands, rocky areas and forests. Results showed that connectivity increased aggregation of areas with high nature values and weighting spread them. Hierarchical analysis was used to find out how nature values changed when the conservation status of the areas were taken into account. The results of hierarchical analysis show that conservation status changed the results a lot. Difference between main analysis and hierarchical analysis was much greater than when taken into account connectivity of feature weights. Hierarchical comparison revealed that many areas with considerable high nature values areas are not presently strictly protected.

Ympäristön- ja luonnonsuojelu Suomessa on muuttunut merkittävästi sotien jälkeen. Puhtaasti luonnontuntemukseen ja harrastuneisuuteen pohjaavan luonnonsuojelun rinnalle on kehittynyt 1960- ja 1970-lukujen vaihteessa politisoitunut ja institutionalisoitunut ympäristönsuojelu. Vielä tuolloin ympäristön muutokset koettiin erillisinä ongelmina, kuten ilman saastumisena, elintarvikkeiden myrkkypitoisuuksina ja maiseman pilaantumisena, mutta yksittäisistä ongelmista alkoi kehittyä laajempi kokonaisuus eli ympäristökysymys. Ympäristökysymys käsittää negatiivisesti koettujen ympäristön muutosten tiedostamisen, problematisoinnin, hallinnan ja politiikat. Vaikka ympäristökysymys muodostui ja ympäristöongelmat tunnistettiin yhteiskunnallisiksi ongelmiksi jo 1960-luvun lopulla, kesti ympäristöhallinnon organisoituminen vuosikymmeniä ja ympäristöministeriö perustettiin vasta vuonna 1983. Tutkimuksessani selvitän, millaisena ympäristökysymyksen kehittyminen näyttäytyy Helsingin Sanomien pilapiirroksissa vuosina 1956-1985. Pilapiirros kertoo enemmän kuin pelkkä uutinen; siitä voi tulkita julkaisuajankohdan asenneilmapiiriä sekä ainakin piirtäjänsä mielipiteen, eli paljon sellaista, jota neutraaliin uutistekstiin ei paineta. Lisäksi se, että pilapiirros on tehty ja julkaistu, paljastaa piirroksessa kuvatun aiheen olevan ajankohtainen ja puhuttava. Tutkimuksen aineistona on Helsingin Sanomien pilapiirrokset vuosilta 1956-1985, jotka ovat kaikki Kari Suomalaisen käsialaa. Ajanjaksolle osuu monia suomalaisen ympäristökysymyksen suuria tapahtumia: yksittäisiä taisteluja, kuten Koijärvi, runsaasti uusien luonnon- ja ympäristönsuojeluun liittyvien lakien säätämisiä sekä ympäristöhallinnon organisaation uudistaminen ja oman ministeriön perustaminen. Tutkimus on yhteiskunnallista ympäristötutkimusta ympäristöhistorian kentällä, ympäristötietoisuuden ja ympäristöliikkeiden historiaan painottuen. Tutkimusote on teorialähtöinen, ja sisällönanalyysi on limittynyt aineistonkeruuvaiheeseen. Lopputuloksena on katselmus siitä, miten ympäristökysymys on kehittynyt Suomessa yksittäisten ympäristöongelmien käsittelystä yhteiskunnalliseksi kysymykseksi

In the years 2017-2019 there was quite vivid discussion about the level of logging in Finland. Finland, as forested country, had put high hopes of bioeconomy as a strategyline for future. In addition the demand of cellulose and other woodproducts had been on rising track and there was plans to build numereous pulp- and bioproduct plants in Finland. Hence the pressure to increase the level of logging had been risen. In the meantime European union tried to achieve more ambitious climatepolicy and conceived a new LULUCF (land use, land-use change and forestry)-act, which allows to take into acount carbon sinks of forests in each of the member country. Aspiration to increase logging and mitigate climate change and preserve forestnature did not go together very well. As a method in this study theory-based content analysis is used, in which thoughts of economic historian Karl Polanyi of self-regulated markets, fictitious commodities and double movement is applied. According to Polanyi self-regulated markets wake protective counter movements in society. These counter reactions are spontanious and they can emerge from various parts of society. They softened the hit caused to people and nature by markets. Polanyi called this mechanism doudle movement. The material contains mediasources such as articles from Helsingin Sanomat and Yleisradio and also public documents of the state. According to this study the markets determinate the use of forests and the level of logging in Finland. Public policy doesn’t want to compromise the strong export industry and employer such as forestry. The use of forest is organized by the rules of markets and tree is now used as a commodity as a timber, even if nature is not originally produced to sale. Finnish forestry is a small part of growth demanding global capitalist system. As counter movements in this case appear environmental organizations, scientists, journalists, politics and citizens. Those groups were worried about carbon sinks and biodiversity of forests. Also European unoion acted as counter movement, when it demanded reduction of emissions of other sectors like housing and transport if carbon sinks of forests decrease. All in all the power of counter movements wasn’t high enough to change the direction of forestry in Finland, which is over the years embedded in finnish society. A potential conflict can still emerge, when traditional nature based industry navigates in the field of international commerce, which resemble Polanyi’s selfregulated markets. There was clear evidence of counter movements and they got more value from the discussion of climate change and the overall greening of society. On the other hand also lumber industry could present them as green, because they use renewable resources, which also replace fossil fuels. The larger impact on the level of logging is system-based pendulumus counter movement, like depression, which swing the world economy.

Perinteisesti ympäristönsuojelussa on kiinnitetty huomiota lähinnä myrkyllisiin yhdisteisiin. Suuret sinällään vaarattomien aineiden materiaalivirrat ovat jääneet huomiotta. Schmidt-Bleek (2002) korostaa näiden materiaalivirtojen merkitystä ja nostaa tavoitteeksi tuotteiden ja palveluiden dematerialisoinnin. Tuotteiden ja palveluiden materiaalienkulutusta mittaamaan on kehitetty Wuppertal-instituutissa MIPS-mittari. MIPS tulee sanoista material input per service-unit eli materiaalipanos palvelusuoritetta kohti. Kuljetuksien materiaalienkulutusta on tutkittu Suomen oloissa vähän ja maailmallakin vain rajoitetusti. Tämän vuoksi kuljetuksien materiaalien kulutusta tutkimaan perustettiin FIN-MIPS-Liikenne -hanke, jonka tarkoituksena on verrata keskenään eri kuljetusmuotoja. Tämän osatutkimuksen tarkoituksena on selvittää raideliikenteen materiaalienkulutus MIPS-laskentamenetelmän avulla. Tarkastelun kohteina olivat Kouvola–Pieksämäki-rata ja rakenteilla oleva Kerava–Lahti-oikorata. Kouvola–Pieksämäki-rata on vanha yksiraiteinen rataosuus, joita on runsaasti Suomen rataverkolla. Kerava–Lahti-oikorata taas edustaa modernia ratarakentamista. Rataosuuksilta poimittiin erilaisia ratatyyppejä: normaalirakenne, kallioleikkaus, silta ja tunneli. Näiden materiaalinkulutus tutkittiin, jotta saataisiin kuva siitä, mitkä rakenteet ovat olennaisia ratainfrastruktuurin materiaalipanoksessa. Myös kaluston materiaalipanos tutkittiin. Tämän lisäksi rataosuuksia tarkasteltiin tapaustutkimuksina. Tällöin tutkittiin myös ratapihojen, varikoiden, konepajojen, asemien, tehtaiden ja radanpidon vaikutus raideliikenteen materiaalipanokseen. Palvelusuoritteena oli henkilö- ja tonnikilometrit, joita tarkasteltiin kolmella erilaisella liikennetasolla. MIPS-luvut laskettiin neljässä eri kategoriassa: abioottiset luonnonvarat, bioottiset luonnonvarat, vesi ja ilma. Bioottiset materiaalipanokset olivat kuitenkin hyvin pieniä ja liittyivät ainoastaan muutamaan kulutettuun aineeseen. Abioottisen materiaalipanoksen muodostumisen kannalta olennaisimpia olivat maarakennustoimenpiteet. Veden kulutus taas koostui suurimmaksi osaksi pois paikoiltaan siirretystä sadevedestä. Ilman kulutuksen muodostumisessa keskeinen merkitys oli sähkönkulutuksella ja rakenteilla, joissa käytettiin runsaasti betonia. Kouvola-Pieksämäki radan materiaalipanos oli kaikissa kategorioissa Kerava-Lahti oikoradan materiaalipanosta alhaisempi, koska Kouvola-Pieksämäki rata on yksiraiteinen ja maarakennustoimenpiteiltään yksinkertaisempi. Kaluston osuus MIPS-luvusta oli alhaisin abioottisessa kategoriassa. Vesi-MIPS-lukuun ja erityisesti ilma-MIPS-lukuun kalusto vaikutti huomattavasti. Kaluston merkitys kasvoi liikenteen vilkastuessa.

Sustainability is a normative concept embedding ethical commitments. A central ethical issue in the sustainability debate and sustainability science regards moral standing. Moral standing is a philosophical concept that means that a being matters morally for their own sake and that there are direct duties owed to the being. It is widely accepted in contemporary ethics that, in addition to humans, at least some sentient nonhuman animals have moral standing. However, the dominant academic and political discourse of sustainability has hitherto focused only on the moral claims of humans without a critical examination of this anthropocentrism. In anthropocentrism, a view of moral standing, only humans have moral standing or they have a much higher moral standing than any nonhumans. Animal and environmental ethicists have questioned anthropocentrism through philosophical arguments. Nevertheless, the academic discourse of sustainability has been disconnected from the philosophical research on moral standing. There is, thus, a research gap in examining moral standing within sustainability science by drawing also on ethical research. This master’s thesis integrates the two distinct fields of knowledge, that is, sustainability science and ethical research on moral standing. The aim is to answer the following research questions: (1) What kind of anthropocentric and non-anthropocentric conceptions of sustainability are there in sustainability literature? (2) What kind of conceptions of sustainability ensue from the main philosophical views of moral standing? (3) How plausible are the different anthropocentric and non-anthropocentric conceptions of sustainability? The thesis applies the philosophical method for investigating the plausibility of alternative views. With animal ethical arguments, I defend the plausibility of a sentiocentric and unitarian conception of sustainability that considers the interests of all sentient beings equally. Also, I present a typology of the main anthropocentric and non-anthropocentric conceptions of sustainability based on philosophical literature on moral standing. My typology characterises the key features of what I call the strong variety of anthropocentric sustainability, the weak variety of anthropocentric sustainability, sentiocentric sustainability, biocentric sustainability and ecocentric sustainability. In addition, this research employs interdisciplinary literature related to the topic and reviews the anthropocentric and non-anthropocentric conceptions of sustainability in sustainability literature. Based on my analysis, I contend that the dominant conceptions of sustainability maintain anthropocentric speciesism, that is, discrimination according to species classification within an anthropocentric worldview. This bias is present, for example, in the conceptions of sustainability of the Brundtland Report, the United Nations’ Agenda 2030, the planetary boundaries framework as well as IPCC reports examined in this thesis. Some non-anthropocentric conceptions of sustainability are starting to emerge in academic discourses: interspecies sustainability, posthuman sustainability, ecocentric sustainability, multispecies sustainability, what I call multicriterial sustainability and defences of the animal ethical dimensions of sustainability. Hitherto, the discourse of sustainability has, still, rarely questioned anthropocentric speciesism. I argue that the anthropocentric conceptions of sustainability lack plausibility for five reasons. Firstly, it is morally wrong to engage in speciesist discrimination. It is wrong to disregard sentient nonhuman animals’ interests and equal duties owed to these creatures in the context of sustainability. Secondly, anthropocentric speciesism is connected to discrimination against certain animalised and marginalised humans, such as indigenous peoples. Thirdly, normative claims require ethical justification, which makes it unacceptable to assume anthropocentrism without critical examination. Sustainability science should consider ethical research on moral standing and aim at overcoming the speciesist bias through critical reflection. Fourthly, from a psychological perspective, it is valuable to oppose oppressive systems that, according to research by Melanie Joy, distance humans from reality and their authentic experience. Fifthly, the sentiocentric equality of all sentient beings protects environment and wellbeing by opposing the animal industry. Also the biocentric and ecocentric conceptions of sustainability lack plausibility, despite their non-anthropocentrism, as only sentient beings have interests. I conclude that there is a duty to embrace the sentiocentric and unitarian conception of sustainability that commits to the equality of all sentient beings, which eliminates discrimination. This conclusion entails a duty to transform the paradigm of sustainability science and the discourse of sustainability. In future research, it is essential to further develop this sentiocentric conception of sustainability, examine its possible challenges and how societies and the academic world could implement it.

In a national assessment of threatened habitat types published in December 2018, the state of brooks in Finland was evaluated poor throughout the country. The situation is the worst in the channels of clay rich areas that have a long history of agriculture and are suffering from excessive nutrient loads. The developmental direction of all flowing water habitat types is negative compared to the assessment given in 2008. Currently there are tens of thousands of kilometers of brooks in the need of restoration.The problem with the already accomplished restorations is that they are limited only to specific and short parts of the channels. Because the restorations usually do not include monitoring, the data available of the effects of the restorations is limited. The problem is global, which has led to only small development in the restoration methods in the past decades. In order to improve the condition of brooks in Finland, there is a need for new planning methods focusing on the drainage basins and the importance of monitoring. Methods developed in Central Europe and North America focus in considering the restoration area and its drainage basin as one unit, mapping of the original characteristics of the ecosystem and specifying the goals of restoration. In the presence of specific goals, the monitoring will become an integral part of the restoration plan. This study investigated the effect of setting specific goals for the restoration, and whether it could offer solutions in improving the situation of brooks in Finland. This study applies to brooks in general, but through the restoration example used, focuses especially in the restoration of channels in agricultural areas. This study describes the first extensive restoration of agricultural brook completed in Finland. Kumianoja, located in Hämeenkoski, was restored to flow in its original meandering shape of the channel in the length of approximately one kilometer. With the documentation of this restoration, this study creates an overview of brook restoration planning and the different stages of its execution. The study was part of the “Sustainable management and restoration of dredged agricultural streams (KURVI)”-project (2016-2018) of the Finnish Environment Institute that was funded by the Ministry of the Environment. The goal of the project was to develop methods for improving the state of the flowing waters damaged by the agricultural land drainage according to the Strategy for Protection and Restoration of Small Water Bodies published in 2015 and the goals of the Water act renewed in 2011.

The insulin/insulin-like growth factor signaling pathway plays an important role in the regulation of growth and development, metabolism, lifespan, reproduction and stress response of an organism. As the pathway is evolutionarily conserved, Drosophila melanogaster serves as a powerful model for characterizing the underlying molecular mechanisms and genetic components, which may further appear as potential therapeutic targets for metabolic disorders. Drosophila has eight genes encoding insulin-like peptides (DILPs), which display stage- and tissue-specific expression patterns. Furthermore, the expression of DILPs is regulated by nutritional status. To date, only few transcription factors have been shown to regulate the expression of the dilp genes: for instance Dachshund and Eyeless are known transcriptional activators of dilp5 and dFOXO is a known transcriptional activator of dilp6. Since transcription factors directly regulating the expression of dilps are not yet well known, there is a need to study them further. The aim of this master’s thesis was to screen for novel transcriptional regulators of the dilp genes and study their phenotypic effects in vivo. For this purpose Drosophila S2 cells were transfected with vectors containing the promoter regions of all the dilp genes and with a library of 822 transcription factors. Promoter activity of the dilp genes was further measured by using the Dual-Luciferase® Reporter Assay System. Some of the revealed potential regulators were further studied in vivo to confirm their regulatory functions by using RNA interference to silence these factors or overexpressing them and by performing quantitative real-time PCR to measure dilp expression levels. Subsequently, organismal growth and development were assessed and effects on larval metabolic profiles were monitored. The screen revealed a few potential transcriptional regulators, among which Spalt-related (Salr) was selected for further study. It was shown to act as an activator of dilp6 expression. dilp6 is an interesting target among the dilp network due to its central role as a communicator between two of the important organs involved in insulin signaling: the brain and the fat body. Subsequently, both Salr and DILP6 were shown to have a role in controlling growth and development of larvae. Based on the experiments in this thesis Spalt-related seems to have a broader role as a metabolic regulator, which is not limited to its function as a regulator of dilp6. Thus, it might act as an important link between the insulin signaling pathway and other pathways regulating growth and metabolism in Drosophila.