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  • Sademies, Jenni (2018)
    In my thesis I analyzed the ongoing discussion about the relationship between economic growth and the environment by the means of content analysis and discourse analysis. As the continuously growing use of natural resources has a connection to many environmental problems, the functioning of the economy is essential when discussing the alternative solutions to environmental problems. I wanted to investigate what the discussion about the relationship between economic growth and the environment includes, and what kind of phenomenon is economic growth represented as. Theoretical frameworks I used were the environmental discourse analyses of Hajer (1995) and Dryzek (2005). I also paid attention to the organizations behind the discourses by classifying the writers due to the organization they represented. The material I used were texts published in the Internet, with no limitations concerning the form of the publication or the publisher. The only limiting factors I used, were the search terms. The material was taken from a database called “Economy and the Environment” (translation from Finnish), and it was not originally collected for this thesis, but for any scientific or educational purposes. I analyzed three discourses in the discussion concerning the relationship of economic growth and the environment: “Belief in growth”, “Green growth” and “Growth critique”. In Belief in growth, economic growth was an intrinsic goal. Pursuit for higher material standard of living was unquestionably a good thing. Belief in growth seemed to be the discourse especially for business interest groups. There were quite many academic writers too, but the academics were not mainly writing in belief in growth discourse, but were also presented in great numbers among the writers of all three discourses. In Green growth discourse economic growth was not an intrinsic goal, but was considered necessary for financing the welfare state. There were hopes that technological development, ecoefficiency and a transformation towards service and information economy would lessen the adverse environmental effects of economic growth. Technology had a major role in Green growth, and it was even seen as a major force itself, solving environmental problems without any role from the users. Amongst the writers of Green growth, Officials and members of the political Green party of Finland were presented in large numbers. In Growth critique discourse endless economic growth was seen impossible on a finite planet, and the ongoing strive for economic growth was seen to cause environmental and social problems. The discourse wanted to change our economic system, so that the pursuit for growth could be abandoned. Abandoning the pursuit for growth was seen also as a question of global equality: the rich countries were hoped to abandon the pursuit for growth, so that the developing countries would have resources left for growth. Compared to other discourses, amongst the writers of Growth critique, NGO’s, social movements, and civilians were presented in large numbers. The discussion about the relationship of economic growth and the environment constituted as a whole in such a way that in the discourses there were expressed opinions about other discourses and their writers. On the other hand, the discussion included many disconnecting elements. This was for example due to the underlying perceptions of environmental problems, and the nature itself, which differed clearly among the three discourses. This finding is convergent with the discourse analyses of Hajer and Dryzek. In Belief in growth environmental problems were local, and mostly described as pollution, which can be removed with the means of cleaning technology. Nature, in this discourse, was a collection of elements which can be used and organized by humans how ever needed. Also in Green growth environmental problems were mostly described as pollution, but the existence of other kinds of environmental problems were recognized as well. In this discourse the global scale existed, as there was a lot of discussion about climate change. In Growth critique environmental problems were seen as one big global crisis, caused by the excessive use of natural resources by humanity. In both, Green growth and Growth critique, nature was seen as a system of which humans are strongly dependent on. The weakest points in the discussion were related to the use of concepts of economics and environmental sciences, and weakly justifiable arguments. In Growth critique, some complicated concepts of economics and environmental sciences were used, which seemed to be poorly understood by the writers of other discourses. Green growth seemed to be suffering of a lack of historical perspective, especially considering the discussion about ecoefficiency, where strong presumptions about the significance of ecoefficiency in reducing the adverse effects of economic growth were made without any historical perspective. In Belief in growth there was incoherence with the concept of economic growth, and means and ends got mixed in the discussion about economic growth and wellbeing. In both Green growth and Belief in growth there were very weakly justified arguments about hopes for information technology and services creating immaterial economy. Interesting topics for future research in Finnish environmental discourse would be the discourse of Green politics, discussion about immaterial economy and the weaknesses in the arguments related to ecoefficiency.
  • Turunen, Iina (2023)
    Finland is committed to sustainable development work, which is guided by the global UN sustainable development action program Agenda2030. The program aims in broad sustainable development work and recognizes cities as key actors in achieving the goals. In Finland, the Commitment2050 online database serves as a tool for achieving the goals of sustainable development. In the website, different actors, including cities and municipalities, commits to operational commitments which require concrete action that promotes sustainability. The purpose of this work is to examine what kind of sustainable development commitments cities and municipalities have made on the “Commitment2050” -website. The thesis aims to create an overall picture of the operational commitments of cities and municipalities. In addition, the thesis highlights what kind of follow-up has been done regarding the commitments and how the actors have evaluated the implementation of the goals of their commitments. The study examined a total of 209 commitments made by cities and municipalities from between the years 2013 and 2022. Monitoring commitments is important so assessment of sustainability implementation can be made. Surprisingly, the study found that a significant proportion of commitments had done no follow-up at all. The actors who had done the monitoring had assessed the fulfillment of the objectives in a very diverse way, which made it difficult to draw conclusions. Thus, deeper knowledge is needed on the commitments implementation.
  • Tuomimaa, Julia (2020)
    Climate change has exacerbated extreme weather events. According to climate scenarios, temperatures will rise and heat periods will become more common also in Finland. These heat periods worsen the urban heat island phenomenon which is known to have negative impacts on human well-being. The impacts of the urban heat island phenomenon can be reduced through careful urban planning, adaptation policies and economic investments. Adaptation to climate change can be monitored through indicators but research and development on adaptation indicators has been largely absent. The development of adaptation monitoring indicators is particularly important in order to ensure the long-term success of the city's adaptation efforts and whether adaptation is moving towards the desired goals. This thesis asks the following research question with the help of a literature review, a focus group discussion and a questionnaire: what kind of indicators can be used to monitor and evaluate adaptation to the urban heat island phenomenon in Helsinki? With the help of the literature review and New York Climate Change Panels framework “the seven steps to indicator selection”, 17 preliminary indicators were developed which were divided into six themes accounting the diverse nature of the urban heat island and its impacts. The indicators were specified in a focus group discussion which had six attendees from environment-, the technical-, social- and health sectors. After the group discussion, the indicators were further modified with the help of a questionnaire answered by ten municipal employees, mainly from the environmental, technical and social and health sectors. On average, respondents found each indicator useful, five indicators were found to be really useful on average, and three indicators were found to be useless among a couple of respondents. After the survey, 11 indicators were modified and one indicator was removed. The end result was 16 indicators for monitoring adaptation to the urban heat island phenomenon divided into the following themes: social vulnerability, state of environment, infrastructure, green-blue infrastructure, policy actions and communication. The development of indicators does not as such contribute to the development of urban adaptation to a desired level, but it is nevertheless an important step in the process of developing adaptation monitoring in the city of Helsinki. This work provides tools for the city to develop their adaptation monitoring strategy which should be systematic and comparable. If the city wants to develop its adaptation monitoring, it is essential to document decisions and programs to explore sufficient coverage. Decisions and programs should be compared against identified adaptation commitments, targets and needs. As a conclusion, this thesis is important step towards more diverse and effective adaptation monitoring. This thesis takes adaptation monitoring forward, developing ways to put adaptation monitoring into practice.
  • Iivonen, Anna-Pauliina (2018)
    Recently, our group reported that mutations in KCNQ1, a potassium channel gene usually linked to long QT syndrome, cause growth hormone deficiency and maternally inherited gingival fibromatosis. Expression of the mutated KCNQ1 with KCNE2 subunit was shown to reduce pituitary hormone secretion in functional experiments in the original study. The aim of this thesis was to investigate if germline mutations in KCNQ1 and KCNE2, a gene encoding an auxiliary potassium channel subunit, could also play a role in the opposite phenomenon, growth hormone excess. Growth hormone (GH) excess causes acromegaly, a condition that is typically due to a GH secreting pituitary adenoma. I screened KCNQ1 and KCNE2 for germline mutations in 45 acromegaly patients by Sanger sequencing and predicted effects of the mutations on protein function by in silico tools. Only deep intronic and synonymous polymorphisms were detected in KCNQ1. These findings were likely insignificant based on the in silico predictions and the variants’ frequencies in the general population. In KCNE2, a heterozygous c.22A>G, p.(Thr8Ala) mutation with an unknown significance was found in two patients. It was present in the general population with a frequency of 0.0038. In conclusion, no evidence of KCNQ1 or KCNE2 mutations being associated with growth hormone excess was found. Mutation screenings of larger patient series and additional functional experiments are needed to shed more light on the roles of KCNQ1 related genes in growth hormone secretion.
  • Mutanen, Justus (2015)
    The International Biology Olympiad is a yearly science competition; hundreds of high school students from over 60 countries take part in it. In Finland, the students are chosen by the national biology competition and the national Olympiad training camp. The research subject of this study is Finnish training for International Biology Olympiad (IBO). About ten high school students who are interested in biology take part at each training camp. Science competitions, such as IBO and training camps, are classified as non-formal or out-of-school science education. Since there is very little scientific knowledge about non-formal biology education, especially about science competitions, the theoretical framework of this study consists of non-formal science education, the relevance of science education and the development of interest. In this study, design research methodology with three research cycles was applied to develop the course. In the study, both theoretical and empirical problem analyses were used. There were two main research questions: 1) What are the needs for the development of the training camp? 2) What kind of training is relevant for the participants? The first research question was elaborated by examining a) what kind of topics of interest the participants have, b) how the participants expect the training to be relevant for them, c) what kind of relevance the previous participants experienced, and d) what kind of effects the previous training camps had on the participants interest in biology and career choice. The features of relevant biology Olympiad training were searched in the second research question. The data was collected from pre-camp and post-camp questionnaires, post-camp interviews and a questionnaire sent for the previous camp participants. The qualitative data was analyzed by content analysis and quantitative data from the questionnaires was analyzed by statistical methods. The main results were following: 1) The biggest needs for development were diversifying the contents and balancing the workload of the camp. The participants of the training camps were interested mostly about medicine and human biology -related topics and cell and molecular biology. In addition, it was found out that the previous participants considered biology education to be individually, vocationally and societally relevant for them. According to them, the training camps were especially individually relevant and had some effects on the career choice. 2) The new training camp for biology Olympiad is especially individually relevant for the participants but it has also some vocational relevance. In the science Olympiad training, special attention should be directed towards developing vocational and societal relevance. A new model for Biology Olympiad training camps was developed based on the collected research data. The individual dimension can be improved by i) diversifying the contents of the camps, ii) including inquiry-based learning modules and by iii) taking a student-centered approach to the development process. The vocational and societal dimensions of relevance can be targeted by iv) allowing the teachers of the participants to take part at camps. The vocational dimension of relevance can be enhanced v) by organizing visits to research laboratories and companies and vi) by enabling the participants to meet university students. The results of this study can be applied to not only to Science Olympiad training but also to non-formal biology education. This research provides models for developing out-of-school biology education and its relevance. This is the first design research study about Biology Olympiad training and it opens up discussion about the relevance of science competitions and Science Olympiad training.
  • Luoto, Senni (2021)
    Kalvojännitteen syntyyn ja sen muutoksiin liittyvät prosessit mielletään usein haastaviksi aiheiksi oppia ja opettaa. Aiheen opetuksesta ja oppimisesta lukiossa ei juurikaan ole tehty tutkimusta, mutta aiemmat tutkimukset yliopisto-opiskelijoilla ovat osoittaneet, että haasteita esiintyy erityisesti lepokalvojännitteen muodostumisen ymmärtämisessä. Aihetta käsitellään suomalaisissa lukiossa pääasiassa ihmisen biologian kontekstissa vapaavalintaisella kurssilla 4 (LOPS 2015) tai 5 (LOPS 2019) ja aihe onkin yksi ihmisen biologian kurssin keskeisistä sisällöistä oppia ja ymmärtää. Kalvojännitteen synty ja sen muutokset on kuvattu suomenkielisissä lukion biologian oppikirjoissa usein yksinkertais-tetusti ja ajoittain virheellisesti. Tämä saattaa johtaa virhekäsitysten syntymiseen, jolloin opiskeltavaa aihetta ei opita riittävällä tasolla. Siksi tämän maisterintutkielman tavoitteena on selvittää, miten solukalvon sähköistä aktiivisuutta opetetaan suomenkielisissä lukioissa ja millaisia virhekäsityksiä opiskelijoilla aiheesta esiintyy. Näiden tulosten pohjal-ta luodaan kehittämistuotos eli opetusmateriaali biologian aineenopettajien käyttöön, jolla voidaan tukea solukalvon sähköisen aktiivisuuden opetusta ja oppimista. Tutkimusmenetelmänä tutkimuksessa käytettiin kehittämistutkimusta, jossa yhdisteltiin teoreettisen ja empiirisen ongelma-analyysin periaatteita. Teoreettisen ongelma-analyysin kautta pyrittiin kartoittamaan lukio-opetuksen kannalta solukalvon sähköiseen toimintaan liittyvät keskeisimmät seikat, joihin monet virhekäsitykset liittyvät, sekä tarkastelemaan niitä eri näkökulmista. Tämä toteutettiin aiemman tutkimuskirjallisuuden avulla. Empiirisessä ongelma-analyysissä tarkasteltiin sekä lukion biologian oppikirjoja (N=3) että kevään 2021 biologian ylioppilaskoevastauksia (N=400) hermosolun aktiopotentiaalin kulkuun liittyen. Molempia aineistoja analysoitiin laadullisen sisällönanalyysin periaatteiden mukaisesti. Teoreettisesta ja empiirisestä ongelma-analyysistä saatujen tulosten perusteella kehitettiin opetusmateriaalin ensimmäinen versio. Opetusmateriaali luetutettiin läpi maisterintutkielman ohjaajilla ja opetusmateriaalia kehitettiin heiltä saatujen kommenttien perusteella. Jatkokehittämisen tuloksena syntyi opetusmateriaalin toinen ja virallinen versio. Tutkimuksen tulokset osoittivat, että solukalvon sähköinen aktiivisuus on haastava aihe sekä opetuksen että oppimi-sen näkökulmasta. Lukion biologian oppikirjoissa esiintyi epätarkkoja kohtia solukalvon sähköiseen aktiivisuuteen liit-tyen ja nämä epätarkkuudet näkyivät opiskelijoiden ylioppilaskokeen vastauksissa yleisinä virhekäsityksinä. Erityisesti esiin nousi virheellinen käsitys natrium-kaliumpumpun ioneja ”palauttavasta” vaikutuksesta aktiopotentiaalin jälkeen. Toinen yleinen virhekäsitys oli, että uusi aktiopotentiaali on mahdollinen vasta kun kalvojännite on hyperpolarisaation jälkeen palautunut lepotilaan. Tulosten perusteella voidaan todeta, että ainakin osa opiskelijoiden virhekäsityksistä on lähtöisin oppikirjoista eikä näitä virhekäsityksiä ole pystytty korjaamaan opettajan toimesta opetustilanteessa. Ylioppi-laskokeessa tehtävään vastanneista opiskelijoista 11,3 % ei osannut vastata tehtävän ensimmäiseen kysymykseen ollenkaan. Vastauksista, joissa opiskelija oli vähintään välttävällä tasolla onnistunut vastaamaan kysymykseen (N=381), 92,4 % sisälsi yhden tai useamman virhekäsityksen. Virhekäsitysten karsimiseksi paras keino on estää niiden syntyminen. Siksi tämän tutkimuksen perusteella voidaan todeta, että lukion uuden opetussuunnitelman biologian oppikirjat tulisi päivittää sellaisiksi, että virheellistä käsitystä ei pääse syntymään. Tämä ei kuitenkaan pelkästään riitä, vaan myös lukion biologian opettajien tulisi luopua vanhoista opetusdioistaan ja varmistaa, että he opettavat aihetta nykytiedon valossa oikein. Tässä apuna toimii tämän tutkimuksen osana kehitetty opetusmateriaali solukalvon sähköisestä aktiivisuudesta hermosolun aktiopotentiaalin kontekstissa. Jotta opetusmateriaalin toimivuutta ja opiskelijoiden käsitteellistä muutosta voidaan arvioida, jatkotutkimukset ai-heesta ovat tarpeellisia.
  • Sarpo, Maija (2008)
    Yhteiskunnalliset liikkeet ovat merkittävä osa poliittista järjestelmäämme. Niillä on kuitenkin usein vajavaiset poliittiset vaikutusmahdollisuudet ja niukat resurssit. Yksi liikkeiden tärkeimmistä poliittisen painostuksen keinoista on mobilisoituvan ihmisjoukon määrä. Tutkimuksessa tarkasteltiin yhden kansalaisliikkeen kautta tämän instrumentin käyttöä ja hallintaa liikkeissä. Tutkimuskohteena oli Itä-Uudellamaalla vuodesta 2006 lähtien toiminut uraanikaivosten vastainen kansalaisliike Uraaniton.org. Tutkimuksessa tarkasteltiin Uraaniton.org:n osallistumista ympäristöongelman määrittelyprosessiin sekä arvioitiin määrittelyn vaikutusta kannatuksen saamiseen ja toiminnan mobilisoitumiseen. Tutkimuksen empiirinen aineisto muodostui Uraaniton.org-kansalaisliikkeen aktiivisten toimijoiden haastatteluista ja liikkeen jäsenten tuottamista teksteistä. Aineisto analysoitiin kvalitatiivisin menetelmin. Teoreettisena viitekehyksenä tutkimuksessa käytettiin kehystämistä. Yhteiskunnallisten liikkeiden tutkimuksen yhteydessä kehystämisellä tarkoitetaan sellaista merkitysten antoa ja tulkintaa tapahtumista ja olosuhteista, jolla pyritään keräämään yleisön kannatusta ja mobilisoimaan potentiaalisia toimijoita sekä asettamaan vastustajien kehystäminen kyseenalaiseksi ja vaikeuttamaan vastapuolen mobilisaatiota. Uraaniton.org-kansalaisliike määritteli Itä-Uudenmaan uraanikaivoshankkeen kansalaisten oikeuksia loukkaavaksi yhteiskunnalliseksi ongelmaksi. Liike koki hankkeen epäoikeudenmukaiseksi, sillä kamppailussa olivat vastakkain ulkomaisen suuryrityksen oikeus harjoittaa liiketoimintaa ja paikallisten ihmisten oikeus turvalliseen ja terveelliseen elinympäristöön. Valtion virkamiesten nähtiin ajavan kaivosyhtiön etuja ja toimivan siten epäoikeudenmukaisesti. Uraaniton.org nosti ilmiön puheenaiheeksi ja käynnisti ongelman määrittelyprosessin. Liike loi konkreettiset puitteet protestille ja yhdisti toimintaan valmiit kansalaiset. Kehystämisellään Uraaniton.org onnistui muodostamaan Itä-Uudellemaalle yhtenäisen protestirintaman. Kohdistamalla toimintansa ainoastaan Itä-Uudenmaan uraanikaivoshankkeen vastustamiseen liike löysi hyvin heterogeeniselle joukolle pienimmän yhteisen nimittäjän, johon kaikkien oli mahdollista samaistua. Uraaniton.org saavutti päätavoitteensa: Itä-Uudenmaan uraanivaltaushakemukset hylättiin. Osana valtakunnallista uraanikaivosten vastaista kansalaisliikehdintää Uraaniton.org vaikutti kaivoslain uudistamiseen, muovasi uraanikaivoksista käytävää keskustelua ja osoitti kaivosyhtiöille, että ainakaan ilman vastarintaa Suomeen ei uraanin etsintää kaivata.
  • Ketola, Katri (2015)
    Darwinian selection can be measured and investigated from gene sequences. A certain gene form favored by positive selection will become more common in the population. Detecting strong positive selection is rare, but it has been found to affect genes involved in immune defense and perception of odorants. Genes under positive selection have a possible role in speciation or adaptation. This is why chemical communication, being based on the sense of smell, is an interesting topic for measuring natural selection and positive selection in particular. Social insects, such as ants, are model organisms for chemical communication. They use chemical communication not only for finding nutrition and detecting intruders, but also in coordinating the activities of several thousands of colony members. Insects perceive odorant signal molecules with their antennae. Odorant binding proteins (OBP) and chemosensory proteins (CSP) bind and transport odorant molecules through the sensillar lymph. OBPs and CSPs are also suspected to have a role in selecting odorants. This work focuses on two OBP genes and two CSP genes to study natural selection. All of these genes are conserved among all ant species. Three of these genes, OBP1, CSP1 and CSP7, are strongly expressed in the antennae suggesting that they function in chemical communication. CSP7 also has a known function in nest mate recognition in ants and OBP1 is known to bind a queen pheromone in the honeybee. The data includes gene sequences from 7 Formica ant species (270 sequences in total). The main goals of the research were to find out 1) the extent of variation between and within closely related ant species, 2) which evolutionary forces, natural selection or random drift, are behind the variation and 3) are there systematic differences between the two social forms of ants suggesting that these genes would affect the social structure of an ant colony. The variation in the sequence data was visualized by phylogenetic, principal coordinate and fixed differences analyses. Differences between populations were studied by FST values. The evolutionary forces shaping chemical communication genes within the species and populations, were studied by McDonald-Kreitman test, Tajima s D, Fu and Li test and MFDM test. The data shows that two of the species, F. cinerea and F. exsecta, significantly differ from the other five ant species, the F. rufa group species, and that the F. rufa group species don t significantly differ from each other based on these genes and this data. This could be due to their recent speciation or crossing between the species leading to hybrids in the data. The results of the evolutionary analyses are inconsistent. However, CSP7 has the strongest indication of selection based on all of the tests. Possible selection and sequence variation was detected at a predicted transcription factor binding site in F. cinerea. This indicates that selection might affect the regulation of CSP7. In the future it would be interesting to check the true transcription factor binding sites experimentally.
  • Silfverberg, Paula (2022)
    Toimiva vesihuolto on yhteiskunnalle elintärkeä. Tärkeimpinä vesihuollon tavoitteina on puhdistaa juomavesi ja vähentää jätevedestä aiheutuvaa ympäristökuormitusta. Kemianteollisuudella on keskeinen rooli tuottaa kemikaaleja vesihuollon käyttöön. Vedenpuhdistus on yksi osa kemikaalien arvoketjua, jossa useat eri toimijat osallistuvat kemikaalien raaka-aineiden valmistukseen, kemikaalien tuottoon, kuljetukseen ja käyttöön, sekä jätteiden loppusijoitukseen. Kemikaalien arvoketjulla on vaikutuksia ympäristöön. Vedenpuhdistuskemikaalien arvoketjua ei ole tutkittu kemikaalien valmistajien ja ostajien välisessä suhteessa. Tutkimuksessani selvitin, kuinka biodiversiteetti näkyy vedenpuhdistuskemikaalien arvoketjun päätöksenteossa ja miten se vaikuttaa kemikaalien ostopäätöksiin. Biodiversiteetti takaa ihmistoimintaakin ylläpitävien ekosysteemipalveluiden toiminnan, kuten veden kiertokulun. Merkittävimpiä uhkia biodiversiteetille ovat vieraslajit, ilmastonmuutos, ravinnekuormitus, ympäristömyrkyt ja roskaantuminen, elinympäristöjen muutokset ja luonnonvarojen liikakäyttö. Kestävillä ratkaisuilla arvoketjun eri toimijat voivat vaikuttaa biodiversiteettiin positiivisesti. Tutkimuksessa haastattelin kuutta vedenpuhdistuslaitoksissa ja kemikaaleja valmistavassa yhtiössä työskentelevää osto- ja myyntipuolen asiantuntijaa puolistrukturoiduilla asiantuntijahaastatteluilla. Lisäksi tarkastelin yhtiöiden vastuullisuusraportteja. Analysoin aineistoa teema-analyysin keinoin. Keskeisimpänä tuloksena havaitsin, että vesikemikaalien arvoketjussa päätettäessä kemikaalien ostamisesta ja myymisestä, toimijat asettavat kemikaalien hinnan ja toimitusvarmuuden ympäristöasioiden edelle. Biodiversiteettiä pidetään tärkeänä, mutta ostopäätöksiin se ei vielä vaikuta. Kemikaalien kehittämiseen ja hankintoihin tarvitaan lisää tietoa biodiversiteettivaikutuksista läpi koko kemikaalien arvoketjun.
  • Huusari, Noora (2020)
    Social insects such as ants live in societies and have a strict division of labor between reproductive and worker castes. A colony can consist of even millions of individuals and the number of queens can vary a lot. Populations where each colony comprises just one or few queens are often called kin structured because the relatedness between nestmates is high. Colonies that have lots of queens and the society lives in many connected nests (polydomy) in are referred to as supercolonies. In these colonies relatedness between individuals is low and the workers represent many genetic lineages. Depending on species and the environment where the colony lives societies can behave aggressively towards individuals from other nests to protect their own nest. Ants must be able to recognize members of their own colony from the intruders to be able to protect the nest. Nestmate recognition is a key element in the interaction between nests and species and makes it possible for the workers in the colony to favour their own nestmates in form of care, defence or food acquisition to gain inclusive fitness benefits. To recognise nestmates ants must be able to sense chemical cues. Ants detect these chemical signals through the proteins expressed mainly in their antennas. In this thesis I studied gene expression of genes related to chemosensation in seven Formica species using the RT qPCR method. My study species were kin structured Formica exsecta, F. pratensis and F. fusca and supercolonial F. truncorum, F. pressilabris, F. cinerea and F. aquilonia. My study genes belong to gene families that code for odorant binding proteins (OBP), chemosensory proteins (CSP) and gustatory reseptors (GRT). I want to find out whether the expression of these genes differs between castes, and whether the caste difference varies between kin structured and supercolonial species. Workers have many tasks in the ant colony and to take care of them, they need to have a sophisticated sensory system. For that reason, I expect to find out that the study genes are expressed more in the worker than the queen caste. In addition, I expect the caste difference in gene expression to be higher in the kin structured species than in the supercolonial species. That is because kin structured species behave more aggressively towards intruders and possibly confront intruders more often than the individuals living in supercolonies. Furthermore, in the supercolonies low relatedness between individuals sometimes lead to conflicts inside the nest. For that reason, I suppose queens of the supercolonies express chemosensory genes more than the queens from the kin structured colonies. Overall expression level was the highest for the OBP and the lowest for GRT. The expression level of CSP was in between these extremes. In accordance with my hypothesis gene expression of OBP and CSP was higher in workers in all the study species. GRT expression was worker biased in six of the seven species. Caste difference in expression of chemosensory genes was similar in kin structured and supercolonial species. The expression level varied between species but did not show a pattern depending on the degree of the polygyny. The study revealed that the expression of OBP and CSP is correlated. My results revealed expected worker biased pattern in the expression. The result might be a consequence of better olfactory or taste abilities in the worker caste compared to queens or it may even be consequence of more sophisticated nestmate recognition skills of the workers. This study reveals valuable information about the gene expression of chemosensory genes related to the recognition system in the ants and awakes many new study questions. Chemical sensory system has been studied a lot in the ants, but in the field of expression studies there is still lot to reveal.
  • Sellman, Oona (2021)
    Several studies have stated that social media has the potential to promote sustainable lifestyles. The purpose of this master’s thesis was to study the contents made by “eco-influencers” who make video blogs about sustainable lifestyles on YouTube. This thesis investigated what topics eco-influencers' videos deal with and what kind of means of argumentation are used. The theoretical background included research on sustainable lifestyles and consumption as well as the potential role of social media in promoting sustainable lifestyles. The study was conducted as a qualitative study and the video material was collected from YouTube according to criteria defined by the researcher. The channels of 20 influencers were selected for the final data. From each channel 1-2 videos were selected. At the end, a total of 39 videos were selected. The material was selectively transcribed, and that text served as the basis for the data-driven content analysis. The results show that the contents of the videos emphasize sustainable consumption and responsibility, especially individual responsibility. However, the videos had only a little content about the three most important aspects of a sustainable lifestyle: housing, mobility, and food. According to eco-influencers, sustainable lifestyles are closely linked to the concept of well-being. Sustainable consumption was considered a very important aspect of sustainable living. Eco-influencers mainly use their own experience, opinions, and comparison as a means of argumentation in their videos. Often the arguments were not substantiated with many scientific references. The results suggest that eco-influencers perceive complex sustainability themes as important but want to communicate them in a way that even laypeople understand. This will also make it easier for more non-experts to approach the matter and change their lifestyles. Justifying arguments with opinions and personal experiences can be based on the personal, even entertaining, format of the video blog, or on the fact that experiential knowledge or expertise is valued in social media. Based on the contents of the videos examined in this study, it seems unlikely that they would contribute to great lifestyle changes.
  • Neuvonen, Samuli (2002)
    Kevyen liikenteen kulkutapaosuus on viimeisten kahden vuosikymmenen aikana jatkuvasti vähentynyt henkilöautoliikenteen kasvaessa. Moottoriliikenteen lisääntymisellä on kuitenkin selviä ekologisia (kuten myös taloudellisia ja sosiaalisia) haittavaikutuksia ja siksi tarpeen olisi pyrkiä toisaalta vähentämään liikkumistarvetta ja toisaalta korvaamaan henkilöautoilua kevyen liikenteen - ja joukkoliikenteen - avulla. Tässä tutkimuksessa selvitetään asiantuntijahaastatteluin niitä tekijöitä, jotka kevyen liikenteen käyttöön ja kulkutapaosuuteen vaikuttavat. Tulosten perusteella kevyestä liikenteestä muodostetaan systeemiajatteluun nojautuva laadullinen malli, joka sisältää kevyen liikenteen käyttöön merkittävimmin vaikuttavat tekijät ja näiden tekijöiden väliset vuorovaikutussuhteet. Tutkimuksessa tehtyjen asiantuntijahaastattelujen perusteella tärkeimmät kevyen liikenteen käyttöön vaikuttavat tekijät ovat yhdyskuntarakenne, kevyen liikenteen infrastruktuurin määrä ja laatu sekä ihmisten asenteet, arvot ja elämäntavat. Yhdyskuntarakenne voisi matkaetäisyyksien puolesta antaa mahdollisuudet kevyen liikenteen käyttöön, kattava kevyen liikenteen verkosto voisi luoda siihen muut ulkoiset mahdollisuudet ja ihmisten myönteinen asennoituminen johtaisi kevyen liikenteen valintaan aina silloin kun se olisi mahdollista. Muita merkittäviä tekijöitä ovat moottoriliikenteen määrät ja nopeudet, Suomen aluerakenne, väestön jatkuva ikääntyminen, talouden ja elinkeinorakenteen kehitys, sää- ja ilmasto-olot sekä joukkoliikenteen palvelutaso. Kaikki mainitut tekijät ovat monimutkaisissa vaikutussuhteissa toisiinsa ja lukuisiin muihin tekijöihin. Talouskehitys vaikuttaa väyläinvestointeihin, aluerakenteeseen ja ihmisten asenteisiin, aluerakenne yhdyskuntarakenteeseen, yhdyskuntarakenne henkilöautoilun ja joukkoliikenteen määriin jne. Näistä vuorovaikutussuhteista muodostuu laadullinen malli, jota voidaan kutsua kevyenliikenteen systeemiksi.
  • Elbadri, Khalil (2020)
    An increased attention has been drawn towards porous silicon (PSi) based materials for biomedical applications, due to their promising features demonstrated through several scientific studies. Here, we further investigated the biological responses of PSi nanoparticles (NPs) with different surface chemistries, including immunomodulatory effects, inflammation mitigation and biocompatibility. In this collaborative study, the PSi NPs were investigated both in vitro and in vivo, using different molecular biology and biochemistry techniques, e.g., qPCR, ELISA, cell sorting and cell viability assays. Our results showed the capabilities of these PSi NPs to relieve the inflammatory conditions, whereas significant decrease was recorded of pro-inflammatory cytokines: TNF-α, IL-1β and IL-6. Likewise, these PSi NPs revealed a considerable consumption aptitude of pro-inflammatory reactive oxygen species molecules. Administrating PSi NPs in an acute liver inflammation (ALI) model, showed no conspicuous influence on cellular viability. Thus, the outcome of this study demonstrates the potential biocompatibility of PSi nanomaterials, in addition to their outstanding features as potential candidates for further incorporating in ALI applications.
  • Asmala, Eero (2008)
    Rehevöityminen on Itämeren näkyvin ympäristöongelma. Itämereen päätyvät typpi- ja fosforivirrat lisäävät perustuotantoa, jonka seurauksena lajisto yksipuolistuu ja pohjalle vajoavan biomassan määrä kasvaa. Pohjan hajotustoiminta kuluttaa laajoilla alueilla vedestä kaiken käytössä olevan hapen, jonka seurauksena vain anaerobinen eliöstö pystyy näillä alueilla selviytymään. Kalan merkitys ihmisravintona kasvaa jatkuvasti. Muun eläintuotannon eettiset kysymykset ja kalaruoan terveellisyys ovat etenkin länsimaissa nostaneet kalan suosiota muiden eläintuotteiden kustannuksella. Yhä suurempi osa kulutetusta kalasta on kasvatettua, myös Suomessa. Kalankasvatuksen kuormitus Suomessa on pienentynyt viimeisen 15 vuoden ajan, pääasiassa rehujen kehityksen myötä. Kalankasvatuksesta aiheutuu kuitenkin edelleen paikallisesti merkittäviä rehevöittäviä ravinnepäästöjä, tuotannon tehokkuudesta huolimatta. Teolliseen ekologiaan sisältyvän teollisen metabolian periaatteiden mukaan teollisten prosessien ainevirtoja tulisi sulkea. Tässä tutkimuksessa tarkasteltiin ainevirta-analyysin avulla Suomen merialueiden kalankasvatusjärjestelmän keskimääräisiä vuotuisia typpi- ja fosforivirtoja vuosien 2004-2006 tuotantotietojen perusteella. Kalankasvatusjärjestelmään tulee typpeä 849 t ja fosforia 118 t kalojen rehusta. Ravinteet sitoutuvat kasvatettavaan kalaan tai kulkeutuvat ympäröivään vesistöön. Kaksi kolmasosaa kalankasvatusjärjestelmän ravinnepäästöistä päätyy kasvatusaltaasta suoraan veteen, kolmasosan sitoutuessa kasvuun. ImPACT-analyysillä todettiin vuosien 1980-2006 välillä kulutustottumusten ja teknologian vaikuttaneen merkittävästi kalankasvatuksen typen ja fosforin aiheuttamaan vesistökuormitukseen, sen sijaan väestön ja varallisuuden muutoksilla ei ollut merkitystä. Tutkimuksessa tarkasteltiin myös mahdollisia muutoksia ravinnevirroissa, jos rehun sisältämä Itämeren valuma-alueen ulkopuolelta peräisin olevasta kalasta tehty kalajauho korvattaisiin Itämeren kalasta tehdyllä kalajauholla. Menetelmällä saavutettaisiin huomattavat vähennykset Itämeren altaaseen päätyvissä ravinnevirroissa: typen osalta vähennys olisi 420 t, fosforin osalta nettokuormitus muuttuisi negatiiviseksi, -10 t. Ravinteet konsentroituisivat intensiivisen kasvatuksen alueille, mutta kyseessä on kustannustehokas keino Suomessa Itämereen kohdistuvan ravinnekuormituksen pienentämiseksi.
  • Koutonen, Heini (2018)
    Globaali väestönkasvu ja lisääntyvä kulutus ovat johtamassa muiden ongelmien ohella luonnonvarojen ehtymiseen ja maa- ja vesiekosysteemien saastumiseen. Eräs tapa ratkaista näitä ongelmia on siirtyä kohti resurssitehokkaampaa kiertotalousperiaatteiden mukaista luonnonvarojen käyttöä. Sekä taloudellisten ohjauskeinojen kautta syntyvät kannustimet että yritysten oma aloitteellisuus ovat keskeisiä kiertotalouden mukaisiin toimintatapoihin siirtyessä. Kokonaisvaltaisen muutoksen edistymistä helpottavat konkreettiset kiertotalouden indikaattorit ja mittaustavat. Ramboll Finland Oy:n ja Luonnonvarakeskuksen luoma alueellinen resurssivirtamalli huomioi kattavasti sekä raha- että materiaalimääräisten resurssien liikkeet tarkastelualueella. Tämän tutkielman tavoitteena on laajentaa kyseistä mallia ottamaan huomioon myös yksittäisen yrityksen näkökulma. Tutkimuksessa noudatettiin konstruktiivista lähestymistapaa, jossa luotiin alueelliseen resurssivirtamalliin uusi ulottuvuus yritystason analyysin muodossa. Keskeisenä menetelmänä käytettiin alueellista ympäristölaajennettua panos-tuotosanalyysiä. Tutkielmassa kehitettiin teoreettinen yritystasolle laajennettu alueellinen resurssivirtamalli, jonka toimintaa testattiin numeerisesti Lapin maakunnan kattavasti käyttäen esimerkkiyrityksenä kuvitteellista paperiteollisuuden yritystä. Muodostettuun malliin kytkettiin kiertotaloutta edistäviä taloudellisia ohjauskeinoja, joita olivat maa-ainesvero, vedenottovero, vedenkäyttövero, sekä arvonlisäverokannan lasku valituilla toimialoilla. Lisäksi tutkittiin yrityksen oma-aloitteista muutosta energianlähteiden käytössä. Mallilla pystyttiin havainnollistamaan, kuinka alueen raha- ja materiaalimääräiset resurssivirrat muuttuvat uuden yrityksen tullessa alueelle. Lisäksi muodostetun mallin avulla pystyttiin tarkastelemaan kiertotaloustoimien vaikutuksia sekä yrityksen että aluetalouden raha- ja materiaalimääräisiin resurssivirtoihin. Tutkielman tulosten mukaan ohjauskeinojen vaikutukset sekä yrityksen että alueen talouteen jäivät varsin pieniksi. Ympäristövaikutusten mallinnuksessa huomattiin, että mallin jatkokehittelyssä tulee tehdä rahamääräisen kokonaistuotannon irtikytkentä ympäristövaikutuksista todenmukaisempien tulosten saamiseksi. Vastaava malli voidaan tutkielmassa käytetyllä menettelyllä muodostaa kuvaamaan haluttua aluetta ja siihen voidaan kytkeä minkä tahansa yrityksen tiedot.
  • Komonen-Kauramäki, Jaana (2020)
    Animals and humans communicate in various ways, using for instance motion, gestures and sounds. Through domestication humans now share the same habitat with a number of species. A centuries-old common history with cats has affected both cats’ behavior and vocalization and possibly human behavior as well. Human speech contains acoustic information beyond the linguistic content. An example of the acoustic structures are fast-repeated, pulsed vocalizations, shown to activate for instance dogs. These structures are thought to be universal in communication with domestic species, possibly for all mammals and birds. Still, not all sounds work the same way for all species. Besides universal structures there are probably species-specific features in calls initiated by humans. We call all domestic animals differently and in a species-specific way, but the common goal is to engage the individual’s attention, and have it approach the caller. People call cats in various ways in different countries, but nevertheless the calls share some common acoustical features. Here, the goal of the study was to find out if people from various language and cultural backgrounds use short, fast-repeated sounds to call cats and to find out similarities in the usage of call sounds higher in frequency than speech. For this, I asked people from various language and cultural background to fill in a questionnaire and give audio samples of a call, instructing them to have an unknown cat approach them. The short, fast-repeated sounds from the calls were extracted and features analyzed using various computer programs. The data were statistically analyzed to find out 1) if the repeated sound frequency spectra differ from the spectra of calls including only speech, 2) the effect of cat ownership and language background to call repetition, 3) the effect of cat ownership and language background to call frequency, and 4) the effect of call origin (inheritance) and experience (cat ownership) to call properties. Altogether 56 adults participated in the study. The subjects were from 19 different countries and represented 16 different native languages. 86% of the calls included fast-repeated, short, pulsed segments. The duration and frequency of the calls differed depending on the repeated sound. The fastest repetition rate was with various clicking sounds. The highest frequency content was with calls including “s” sound, such as “pss” and “kss”, and various lip-smacking sounds. While there were differences in the repeated segments depending on the language background of the caller, the common pattern was that the spectral center of gravity in sound frequency was higher for the fast-repeated, short segments than for calls including only speech. In summary, human-to-cat calls have a high frequency content and include short, fast-repeated sound segments regardless of the language background of the caller, in order to get cat’s attention and have it approach the caller. For the follow-up study I suggest expanding the subject population to different language backgrounds. In addition, the call sounds should be compared to predator sounds. Further, the effectiveness of the call sounds could be tested with cats or kittens.
  • Viitala, Katarina (2019)
    Diet has a significant impact on the environment. 25% of Finnish household greenhouse gas emissions are due to food production and food consumption related activities. There is a continuing discussion about the environmental consequences of human nutrition, but the ecological consequences of pets are not nearly as much discussed. Pets are rarely taken into account in food consumption calculations, although they can be a big and central part of the household and its consumption. The aim of this master’s thesis is to focus on the nutrition of the second most popular pet in Finland, the cat, with an environmental perspective. This study examines the environmental impact of cat food from the perspective of the cat food industry and the consumers' choices. Important topics are the origin of food, trends in the cat food industry and consumers’ attitudes and practices. The aim is also to find ways to reduce the environmental impact of cat food. The aim of this work is to (i) examine cat food production and consumption from an environmental point of view, and (ii) examine the attitudes and customs of Finnish cat owners from the point of view of the environmental impact that concerns cat food. The goal is to get a clear picture of the different dimensions of the environmental impact of cat food. To get up-to-date information of the cat food industry, theme interviews were arranged, in April 2016, for two of the largest pet food manufacturers in Finland. In the interviews, important topics like the origin of the meat, import, food packaging and the future of the cat food industry, were brought up. The most important result was the knowledge that the cat food industry uses byproducts from the meat industry, as the animal-based ingredient of cat food. Most of the cat food consumed in Finland is produced all over Europe and domestic cat food production is very small. Cat owners' attitudes and interest in more environmentally friendly cat food and their current environmental practices were surveyed in a short online survey conducted in June-July 2018. The survey was distributed on social media, in groups focusing on cats and cat ownership. The survey gathered a total of 144 responses. The results of the survey suggest that cat owners have an interest in environmentally friendly products and also domestically produced food. However, cat owners were not ready favor environmentally friendly food if the nutritional value, taste, ingredients etc. are not at the same level as the respondent's current choice of cat food. The current practices of cat owners are rather environmentally friendly, as most of them try to recycle the food packages and take into account the amount and the recyclability of packaging materials when choosing cat food. The most important result of the whole study is that both cat owners and cat food manufacturers have a mostly positive attitude towards environmental friendliness and manufacturers in particular are aiming to become more environmentally friendly in the future. For the future, it is essential that manufacturers and cat owners interact with each other on topics about environmental issues and that more environmentally friendly alternatives are developed.
  • Harmoinen, Katri (2019)
    New Zealand is an isolated landmass laying in the Southwest Pacific waters, far away from any major islands or continents. It was the last major landmass to be colonized by people, discovered by the first Polynesian explorers around a thousand years ago. Historically, New Zealand lacked all native mammals (apart from three species of bats) and so has developed a plethora of bird species and other endemic wildlife. The absence of mammalian predators, combined with the continuous isolation for millions of years, has led the evolution of some very unique and charismatic species. One of these species is the iconic symbol of New Zealand – the kiwi (Apteryx spp). The biggest challenge to the New Zealand wildlife has been the introduction of mammalian species to the New Zealand ecosystem. There are 25 species of introduced mammals in New Zealand today that are regarded as pests. The devastation caused by these species is the main cause for the dramatic decline of the endemic New Zealand wildlife, including the iconic kiwi. Nationally, kiwi continue to decline by more than 2% annually and there are estimates of the species going extinct from the wild within 50 years. Since the first more permanent human settlement, more than 50% of the New Zealand breeding birds have gone extinct. In this thesis, the relation between kiwi and introduced mammalian species around the township of Whakatāne, New Zealand, was studied. During summer 2018-2019, three out of eight monitored kiwi chicks were predated by a suspected mustelid/mustelids and DNA swabs were obtained from the bite sites. Volunteer pest trappers were then asked to bring in all their catches in an attempt to catch the individual/individuals responsible for the predations. Molecular tools including microsatellites were used to create ID profiles in an attempt to match the profiles to those obtained from the kiwi chicks. In the second part of the study, the stoats’ stomachs were analysed as part of a diet study. A new, kiwi specific DNA probe was trialled and the remaining stomach contents were sequenced for other native wildlife species. Out of the three predated kiwi chicks, all of them were confirmed to be stoat predations. Unfortunately, none of the stoat ID profiles obtained matched the profile of the kiwi chick Ranui who was the only chick a good micro-satellite profile was obtained for. This confirmed that the stoat/stoats responsible for the predation of Ranui was not caught as part of this study. In the diet part of this thesis, we trialled the kiwi specific probe but could not identify any kiwi DNA in the stoat stomach contents. The DNA sequencing however revealed five other species: tomtit (lat. Petroica macrocephala, 100%), common chaffinch (lat. Frigilla coelebs, 100%), tui (lat. Prosthemadera novaseelandiae, 96%), European hare (lat. Lepus europaeus,100%) and copper skink (lat. Cyclodina aenea, 100%). These findings shed new light on the extent introduced mammalian species contribute to the species loss taking place in the New Zealand forests today. The use of molecular techniques and tools in conservation offers an often faster, cost-efficient and more reliable alternative to traditional monitoring methods of introduced species. The rapid development of these tools has seen New Zealand taking critical steps towards one day becoming predator free. The ambitious goal to rid New Zealand of target introduced species (mustelids, possums and rats) by year 2050 (Predator Free 2050), has been compared as the New Zealand equivalent of putting the man on the moon.
  • Nordling, Emilia (2010)
    During the last century increased emissions of greenhouse gases have caused global climate warming. While the temperatures are still rising, the largest increases have been observed in spring-time temperatures. This may affect the phenology of multiple species. If the increase in temperature affects different species differently, then the lifecycles of various species may become unsynchronized. For interacting species, a disturbance like this may be catastrophic: for example, if the phenology of host plants is drastically altered, then many herbivores may be left without food. Previous research on the effects of climate change on interspecific interactions has focused on bitrophic interactions. My research expands this past emphasis to a tritrophic level: to interactions between plants (penduculate oak, Quercus robur), herbivorous insects (moths: Tischeria ekebladella, Phyllonorycter quercifoliella and P. harrisella) and parasitoids (wasps in the family Eulophidae). Since bud burst in oaks varies significantly among individuals, I also investigated how host genotype may affect the synchrony between species. My hypotheses were that higher spring-time temperatures will disrupt the synchrony between species, and that this disturbance will affect the growth and reproduction of species at higher trophic levels. I also posited that the effects will vary with the genotype of the host. I tested these hypotheses in a field experiment running from April to September 2009. To manipulate temperatures I used a greenhouse, transplanting multiple oak-specific moth and parasitoid species to oaks of different genotypes growing inside and outside of the greenhouse. During the field experiment, the temperature differed by 3.19°C between the interior and the outside of the greenhouse. As a result, the phenology of all species was advanced in the greenhouse interior as compared to ambient conditions. Interspecific synchrony was affected differently in different species pairs: Inside of the greenhouse, the synchrony between oaks and Phyllonorycter moths and between oaks and parasitoids was decreased. The synchrony between oaks and the moth T. ekebladella remained unaffected. Likewise, the synchrony between moths and parasitoids was left intact. The larvae of T. ekebladella grew bigger inside of the greenhouse than outside of it. In addition, a second generation of T. ekebladella occurred inside of the greenhouse, but not under ambient temperatures. Host plant phenology had a significant effect on associated insects: moth larvae of T. ekebladella grew bigger on oaks sprouting leaves early in the season. This increase in performance suggests that moth larvae prefer old leaves. In summary, my study shows that climate change may affect different species and different interspecific interactions in highly different ways. My study also upsets the previous view that mature oak foliage would offer food of inferior quality to moth larvae. In addition, it depicts host plant genotype as a less prominent determinant of insect performance than has been previously assumed.